| http://purl.uniprot.org/citations/3011795 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3011795 | http://www.w3.org/2000/01/rdf-schema#comment | "Insulin-like growth factor (IGF) I receptor was purified from Triton X-100-solubilized human placental membranes by wheat germ agglutinin-Sepharose chromatography followed by immunoaffinity chromatography using alpha IR-3, a monoclonal antibody directed against the IGF-I receptor. Purification of 3200-fold and 2800-fold was achieved from wheat germ agglutinin-Sepharose eluates with regard to IGF-I binding and kinase activities. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions revealed two major protein bands corresponding to the alpha and beta subunits of the receptor, which accounted for at least 90% of the protein content. The purified receptor bound 10-20 micrograms of IGF-I/mg of protein and was more than 95% free of contamination by insulin receptor. It sedimented in glycerol gradients as a single species with a sedimentation coefficient of 13.7 S and gave three protein bands with Mr = approximately 300,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions, indicating that alpha 2 beta 2 is an intact form of the IGF-I receptor. The purified receptor, when incubated with [gamma-32P] ATP, became phosphorylated at tyrosine residues of its beta subunit. This was stimulated 3-fold by IGF-I. It also had IGF-I-stimulated tyrosine kinase activity (5264 pmol of 32P incorporated/min/mg of protein) toward a synthetic peptide corresponding to the autophosphorylation site of pp60src. These data strongly suggest that it is a tyrosine-specific protein kinase."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)57454-3"xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/author | "Jacobs S."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/author | "LeBon T.R."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/author | "Fujita-Yamaguchi Y."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/author | "Kathuria S."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/author | "Cuatrecasas P."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/pages | "7685-7689"xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/title | "Purification of insulin-like growth factor I receptor from human placental membranes."xsd:string |
| http://purl.uniprot.org/citations/3011795 | http://purl.uniprot.org/core/volume | "261"xsd:string |
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