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http://purl.uniprot.org/citations/30119151http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30119151http://www.w3.org/2000/01/rdf-schema#comment"qPCR and FISH in breast cancer.";

Background/aim

The aims of this study were to detect CCND1 , C-MYC , and FGFR1 amplification using qPCR, confirmation with FISH, and to further assess their clinicopathological relevance.

Materials and methods

Thirty-five breast tumor samples were analyzed for amplification of the selected genes using modified SYBR Green qPCR. The accuracy of the qPCR was assessed by FISH as a gold-standard method.

Results

CCND1 , C-MYC , and FGFR1 amplifications were observed in 34.28%, 28.57%, and 17.14% of the 35 samples, respectively. qPCR results were significantly confirmed by FISH and qPCR and FISH showed excellent correlation (P = 0.000). CCND1 amplification with tumor stage (P = 0.044), positive metastatic status (P = 0.042), positive family history (P = 0.042), and C-MYC status (P = 0.005); C-MYC amplification with tumor size (P = 0.021), tumor grade (P = 0.018), tumor stage (P = 0.032), and FGFR1 status (P < 0.000); and FGFR1 amplification with tumor size (P = 0.041) and positive ER status (P = 0.042) were statistically associated.

Conclusion

Our findings revealed that the applied qPCR approach could precisely quantify the relative gene copy number. More studies with a larger sample size are suggested to confirm the clinicopathological value of CCND1 , C-MYC , and FGFR1 amplification."xsd:string
http://purl.uniprot.org/citations/30119151http://purl.org/dc/terms/identifier"doi:10.3906/sag-1710-93"xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/author"Mehdipour P."xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/author"Azarnezhad"xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/author"Javan"xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/author"Tabrizi"xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/name"Turk J Med Sci"xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/pages"759-767"xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/title"Detection of CCND1 , C-MYC , and FGFR1 amplification using modified SYBR Green."xsd:string
http://purl.uniprot.org/citations/30119151http://purl.uniprot.org/core/volume"48"xsd:string
http://purl.uniprot.org/citations/30119151http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30119151
http://purl.uniprot.org/citations/30119151http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30119151
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