| http://purl.uniprot.org/citations/30120927 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30120927 | http://www.w3.org/2000/01/rdf-schema#comment | "Metazoan eggs have a specialized coat of extracellular matrix that aids in sperm-egg recognition. The coat is rapidly remodeled after fertilization to prevent polyspermy and establish a more permanent barrier to protect the developing embryo. In nematodes, this coat is called the vitelline layer, which is remodeled into the outermost layer of a rigid and impermeable eggshell. We have identified three key components of the vitelline layer structural scaffold - PERM-2, PERM-4 and CBD-1, the first such proteins to be described in the nematode C. elegans. CBD-1 tethered PERM-2 and PERM-4 to the nascent vitelline layer via two N-terminal chitin-binding domains. After fertilization, all three proteins redistributed from the zygote surface to the outer eggshell. Depletion of PERM-2 and PERM-4 from the scaffold led to a porous vitelline layer that permitted soluble factors to leak through the eggshell and resulted in embryonic death. In addition to its role in vitelline layer assembly, CBD-1 is also known to anchor a protein complex required for fertilization and egg activation (EGG-1-5/CHS-1/MBK-2). We found the PERM complex and EGG complex to be functionally independent, and structurally organized through distinct domains of CBD-1. CBD-1 is thus a multifaceted regulator that promotes distinct aspects of vitelline layer assembly and egg activation. In sum, our findings characterize the first vitelline layer components in nematodes, and provide a foundation through which to explore both conserved and species-specific strategies used by animals to build protective barriers following fertilization."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ydbio.2018.08.005"xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Moresco J.J."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Yates J.R."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Diedrich J.K."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Prieto J.A."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Olson S.K."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Harrison M.C."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Partida D."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Gonzalez D.P."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Lamb H.V."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/author | "Wilson Z.T."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/name | "Dev Biol"xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/pages | "288-300"xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/title | "CBD-1 organizes two independent complexes required for eggshell vitelline layer formation and egg activation in C. elegans."xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://purl.uniprot.org/core/volume | "442"xsd:string |
| http://purl.uniprot.org/citations/30120927 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30120927 |
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