| http://purl.uniprot.org/citations/30184072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30184072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30184072 | http://www.w3.org/2000/01/rdf-schema#comment | "Sex determination and differentiation are complex processes. As a juvenile hermaphrodite or undifferentiated gonochorist, zebrafish undergo a special juvenile ovarian phase during sex differentiation, making it an excellent model for studying early oogenesis and folliculogenesis. We provide lines of evidence at morphological, molecular, and genetic levels for roles of factor in the germline α (Figla), an oocyte-specific transcription factor, in early zebrafish gonadogenesis. As in mammals, Figla/figla was also expressed in the gonads and its expression in the ovary was also restricted to early oocytes. Disruption of figla gene by CRISPR/Cas9 led to an all-male phenotype in the mutant. Detailed analysis of early gonadal development showed that the germ cells in the mutant were clustered in cysts and underwent meiosis, forming oocytes at prefollicular chromatin nucleolar (CN) stage (stage IA). However, the subsequent transition from cystic CN oocytes to individual follicular perinucleolar oocytes (stage IB) was blocked, resulting in an all-male phenotype in the mutant. The phenotype of figla mutant could not be rescued by estrogen treatment, in contrast to cyp19a1a mutant, and introduction of tp53 mutation also had no effect, unlike in fancd1 and fancl mutants. Transcriptome analysis revealed that many biological processes and pathways related to germ cell development, especially oogenesis, were upregulated in the presence of Figla and that the regulation of figla expression may involve heat shock proteins. Our results strongly suggest important roles for Figla in juvenile ovary development, especially the formation of individual follicles from cystic oocytes."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.org/dc/terms/identifier | "doi:10.1210/en.2018-00648"xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Chen W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Chen W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Song W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Song W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Ge W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Ge W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Qin M."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Qin M."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Wong Q.W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Wong Q.W."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Shirgaonkar N."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/author | "Shirgaonkar N."xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/pages | "3699-3722"xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/pages | "3699-3722"xsd:string |
| http://purl.uniprot.org/citations/30184072 | http://purl.uniprot.org/core/title | "Roles of Figla/figla in Juvenile Ovary Development and Follicle Formation During Zebrafish Gonadogenesis."xsd:string |