| http://purl.uniprot.org/citations/30242097 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30242097 | http://www.w3.org/2000/01/rdf-schema#comment | "Activation of platelet glycoprotein IIb-IIIa (GPIIb-IIIa; integrin αIIbβ3) leads to high-affinity fibrinogen binding and platelet aggregation during hemostasis. Whereas GTP-bound Rap1 GTPase promotes talin 1 binding to the β3 cytoplasmic domain to activate platelet GPIIb-IIIa, the Rap1 effector that regulates talin association with β3 in platelets is unknown. Rap1 binding to the talin 1 F0 subdomain was proposed to forge the talin 1-Rap1 link in platelets. Here, we report a talin 1 point mutant (R35E) that significantly reduces Rap1 affinity without a significant effect on its structure or expression. Talin 1 head domain (THD) (R35E) was of similar potency to wild-type THD in activating αIIbβ3 in Chinese hamster ovary cells. Coexpression with activated Rap1b increased activation, and coexpression with Rap1GAP1 reduced activation caused by transfection of wild-type THD or THD(R35E). Furthermore, platelets from Tln1R35E/R35E mice showed similar GPIIb-IIIa activation to those from wild-type littermates in response to multiple agonists. Tln1R35E/R35E platelets exhibited slightly reduced platelet aggregation in response to low doses of agonists; however, there was not a significant hemostatic defect, as judged by tail bleeding times. Thus, the Rap1-talin 1 F0 interaction has little effect on platelet GPIIb-IIIa activation and hemostasis and cannot account for the dramatic effects of loss of Rap1 activity on these platelet functions."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.org/dc/terms/identifier | "doi:10.1182/bloodadvances.2018020487"xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Sun H."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Ginsberg M.H."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Gingras A.R."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Goult B.T."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Bergmeier W."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Shattil S.J."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Paul D.S."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Lopez-Ramirez M.A."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Lagarrigue F."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Cuevas M.N."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/author | "Valadez A.J."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/name | "Blood Adv"xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/pages | "2358-2368"xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/title | "Rap1 binding to the talin 1 F0 domain makes a minimal contribution to murine platelet GPIIb-IIIa activation."xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://purl.uniprot.org/core/volume | "2"xsd:string |
| http://purl.uniprot.org/citations/30242097 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30242097 |
| http://purl.uniprot.org/citations/30242097 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30242097 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JDL9-mappedCitation-30242097 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30242097 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JE52-mappedCitation-30242097 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30242097 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JED9-mappedCitation-30242097 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30242097 |
| http://purl.uniprot.org/uniprot/#_A0A1L1SVA8-mappedCitation-30242097 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30242097 |