| http://purl.uniprot.org/citations/3024334 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3024334 | http://www.w3.org/2000/01/rdf-schema#comment | "The presence of amplified sequences in mammalian DNA can be determined by in-gel renaturation of labeled restriction digests of total genomic DNA, provided that such sequences comprise at least 20-30 copies per haploid human or hamster genome or 40-50 copies per mouse genome. To detect amplified DNA in mouse cells at a lower level of amplification, a new procedure has been developed. This procedure combines in-gel DNA renaturation with Southern hybridization using a cloned probe containing a short interspersed repeated element (SINE). Using mouse cell lines containing amplified dihydrofolate reductase (DHFR) or c-Ki-ras genes as a model system, and the cloned B2 repeated sequence as a SINE probe, we have shown that this technique can detect gene amplification at a level as low as 10-15 copies of amplified DNA per haploid mouse genome. In-gel renaturation-SINE hybridization was used to assay for DNA amplification in different tissues and in different mouse strains. No tissue-specific DNA amplification has been detected, but comparison of B2-containing repeated fragments between three inbred mouse lines revealed strain-specific polymorphism."xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf01671946"xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/author | "Fukumoto M."xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/author | "Roninson I.B."xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/name | "Somat Cell Mol Genet"xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/pages | "611-623"xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/title | "Detection of amplified sequences in mammalian DNA by in-gel renaturation and SINE hybridization."xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/3024334 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3024334 |
| http://purl.uniprot.org/citations/3024334 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3024334 |
| http://purl.uniprot.org/uniprot/#_Q9D6F9-mappedCitation-3024334 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/3024334 |
| http://purl.uniprot.org/uniprot/Q9D6F9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/3024334 |