| http://purl.uniprot.org/citations/30249303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30249303 | http://www.w3.org/2000/01/rdf-schema#comment | "Spinocerebellar ataxia type 14 (SCA14) is a subtype of the autosomal dominant cerebellar ataxias that is characterized by slowly progressive cerebellar dysfunction and neurodegeneration. SCA14 is caused by mutations in the PRKCG gene, encoding protein kinase C gamma (PKCγ). Despite the identification of 40 distinct disease-causing mutations in PRKCG, the pathological mechanisms underlying SCA14 remain poorly understood. Here we report the molecular neuropathology of SCA14 in post-mortem cerebellum and in human patient-derived induced pluripotent stem cells (iPSCs) carrying two distinct SCA14 mutations in the C1 domain of PKCγ, H36R and H101Q. We show that endogenous expression of these mutations results in the cytoplasmic mislocalization and aggregation of PKCγ in both patient iPSCs and cerebellum. PKCγ aggregates were not efficiently targeted for degradation. Moreover, mutant PKCγ was found to be hyper-activated, resulting in increased substrate phosphorylation. Together, our findings demonstrate that a combination of both, loss-of-function and gain-of-function mechanisms are likely to underlie the pathogenesis of SCA14, caused by mutations in the C1 domain of PKCγ. Importantly, SCA14 patient iPSCs were found to accurately recapitulate pathological features observed in post-mortem SCA14 cerebellum, underscoring their potential as relevant disease models and their promise as future drug discovery tools."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.org/dc/terms/identifier | "doi:10.1186/s40478-018-0600-7"xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Cowley S.A."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Nemeth A.H."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Talbot K."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Becker E.B.E."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Watson L.M."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Ansorge O."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Fuller G."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Vowles J."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Hoekstra S.D."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/author | "Wong M.M.K."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/name | "Acta Neuropathol Commun"xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/pages | "99"xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/title | "Neurodegeneration in SCA14 is associated with increased PKCgamma kinase activity, mislocalization and aggregation."xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/30249303 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30249303 |
| http://purl.uniprot.org/citations/30249303 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30249303 |
| http://purl.uniprot.org/uniprot/#_B2R5T1-mappedCitation-30249303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30249303 |
| http://purl.uniprot.org/uniprot/#_A0A804HIU5-mappedCitation-30249303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30249303 |
| http://purl.uniprot.org/uniprot/#_B7Z3V9-mappedCitation-30249303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30249303 |
| http://purl.uniprot.org/uniprot/#_B7Z3W6-mappedCitation-30249303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30249303 |
| http://purl.uniprot.org/uniprot/#_B7Z870-mappedCitation-30249303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30249303 |