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http://purl.uniprot.org/citations/30249887http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30249887http://www.w3.org/2000/01/rdf-schema#comment"

Aim of study

Recent studies have suggested neprilysin (NEP) play a key role in cigarette smoke-induced nonsmall-cell lung carcinoma; however, the detailed mechanism was still unclear. Here, we employed in vitro human bronchial epithelial BEAS-2B cells to investigate whether and how NEP involved in cigarette smoke condensate (CSC)-induced cancer occurrence.

Materials and methods

In vitro MTT and transwell assay was applied. Live cell imaging and staining were also employed.

Results

In vitro data showed that CSC could increase BEAS-2B cell migration while NEP shRNA could block CSC-induced BEAS-2B cell hypermigration. By biotination and live cell staining, we found that after CSC treatment, cell surface NEP was increased while internalization trafficking was shifted from late endosome/lysosome pathway to recycling pathway. Finally, we found that surface NEP could bind to p120 catenin (p120ctn) for lysosome destination turnover while CSC treatment could change p120ctn membrane/cytosome distribution. Loss of p120ctn will subsequently change NEP trafficking and finally, increase its membrane distribution with a phenocopy manner as CSC.

Conclusion

These data indicated under CSC treatment; losing of membrane p120ctn could upregulate surface NEP protein level and thus facilitate BEAS-2B cell migration."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.org/dc/terms/identifier"doi:10.4103/0973-1482.183182"xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/author"Li D."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/author"Sun L."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/author"Yang K."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/author"Zhang C."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/author"Hong X."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/name"J Cancer Res Ther"xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/pages"S680-S687"xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/title"Cigarette smoke condensate could promote human bronchial epithelial BEAS-2B cell migration through shifting neprilysin trafficking."xsd:string
http://purl.uniprot.org/citations/30249887http://purl.uniprot.org/core/volume"14"xsd:string
http://purl.uniprot.org/citations/30249887http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30249887
http://purl.uniprot.org/citations/30249887http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30249887
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