| http://purl.uniprot.org/citations/30256431 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30256431 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundProstate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is an important diagnostic and therapeutic target in prostate cancer. PSMA/GCPII is also expressed in many healthy tissues, but its function has only been established in the brain and small intestine. Several research groups have attempted to produce PSMA/GCPII-deficient mice to study the physiological role of PSMA/GCPII in detail. The outcomes of these studies differ dramatically, ranging from embryonic lethality to production of viable PSMA/GCPII-deficient mice without any obvious phenotype.MethodsWe produced PSMA/GCPII-deficient mice (hereafter also referred as Folh1-/- mice) by TALEN-mediated mutagenesis on a C57BL/6NCrl background. Using Western blot and an enzyme activity assay, we confirmed the absence of PSMA/GCPII in our Folh1-/- mice. We performed anatomical and histopathological examination of selected tissues with a focus on urogenital system. We also examined the PSMA/GCPII expression profile within the mouse urogenital system using an enzyme activity assay and confirmed the presence of PSMA/GCPII in selected tissues by immunohistochemistry.ResultsOur Folh1-/- mice are viable, breed normally, and do not show any obvious phenotype. Nevertheless, aged Folh1-/- mice of 69-72 weeks exhibit seminal vesicle dilation, which is caused by accumulation of luminal fluid. This phenotype was also observed in Folh1+/- mice; the overall difference between our three cohorts (Folh1-/- , Folh1+/- , and Folh1+/+ ) was highly significant (P < 0.002). Of all studied tissues of the mouse urogenital system, only the epididymis appeared to have a physiologically relevant level of PSMA/GCPII expression. Additional experiments demonstrated that PSMA/GCPII is also present in the human epididymis.ConclusionsIn this study, we provide the first evidence characterizing the reproductive tissue phenotype of PSMA/GCPII-deficient mice. These findings will help lay the groundwork for future studies to reveal PSMA/GCPII function in human reproduction."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.org/dc/terms/identifier | "doi:10.1002/pros.23717"xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Konvalinka J."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Kasparek P."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Sacha P."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Sedlak F."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Zamecnik J."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Maly M."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Sramkova K."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/author | "Vorlova B."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/name | "Prostate"xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/pages | "126-139"xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/title | "A novel PSMA/GCPII-deficient mouse model shows enlarged seminal vesicles upon aging."xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://purl.uniprot.org/core/volume | "79"xsd:string |
| http://purl.uniprot.org/citations/30256431 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30256431 |
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| http://purl.uniprot.org/uniprot/#_O35409-mappedCitation-30256431 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30256431 |
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| http://purl.uniprot.org/uniprot/O35409 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30256431 |