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http://purl.uniprot.org/citations/3026906http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3026906http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3026906http://www.w3.org/2000/01/rdf-schema#comment"The Bacillus sphaericus gene encoding penicillin V amidase, which catalyzes the hydrolysis of penicillin V, has been characterized. The entire nucleotide sequence of the coding region, as well as 5'- and 3'-flanking regions, was determined using an improved sequencing strategy. The deduced amino acid sequence suggests a protein consisting of 338 residues with an Mr of 37,500. The ATG initiator codon is preceded by a putative ribosome-binding site, typical for genes of Gram-positive origin. High expression of the gene was obtained in Escherichia coli using an inducible promoter, showing that the gene product is stable in this heterologous host."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(86)90252-0"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(86)90252-0"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/author"Uhlen M."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/author"Uhlen M."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/author"Olsson A."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/author"Olsson A."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/date"1986"xsd:gYear
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/date"1986"xsd:gYear
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/pages"175-181"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/pages"175-181"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/title"Sequencing and heterologous expression of the gene encoding penicillin V amidase from Bacillus sphaericus."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/title"Sequencing and heterologous expression of the gene encoding penicillin V amidase from Bacillus sphaericus."xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/volume"45"xsd:string
http://purl.uniprot.org/citations/3026906http://purl.uniprot.org/core/volume"45"xsd:string
http://purl.uniprot.org/citations/3026906http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/3026906
http://purl.uniprot.org/citations/3026906http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/3026906
http://purl.uniprot.org/citations/3026906http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/3026906
http://purl.uniprot.org/citations/3026906http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/3026906
http://purl.uniprot.org/uniprot/P12256http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/3026906
http://purl.uniprot.org/uniprot/#_P12256-citation-3026906http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/3026906