| http://purl.uniprot.org/citations/30275529 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30275529 | http://www.w3.org/2000/01/rdf-schema#comment | "CRISPR-mediated base editing can introduce single-nucleotide changes in the DNA of living cells. One intriguing application of base editing is to screen pivotal amino acids for protein function in vivo; however, it has not been achieved. Here, we report an enhanced third-generation base-editing system with extra nuclear localization sequences that can efficiently introduce a homozygous base mutation in embryonic stem cells. Meanwhile, we establish a strategy to generate base-mutant mice by injection of haploid embryonic stem cells carrying a constitutively expressed enhanced third-generation base-editing system (4B2N1) and single guide RNA into oocytes. Moreover, transfection of 4B2N1 cells with a single guide RNA library targeting the Dnd1 gene allows one-step generation of mutant mice with a base mutation. This enables the identification of four missense mutations that completely deplete primordial germ cells through disruption of DND1 protein stability and protein-protein interaction. Thus, our strategy provides an effective tool for in vivo screening of amino acids that are crucial for protein function."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41556-018-0202-4"xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Chen Y."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Ding Y."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Huang S."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Lu L."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Lou X."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Sun Z."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Shi H."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Tang W."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Yang S."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Yin Q."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/author | "Yan M."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/name | "Nat Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/pages | "1315-1325"xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/title | "CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development."xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/30275529 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30275529 |
| http://purl.uniprot.org/citations/30275529 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30275529 |