Results

RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/30280777http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30280777http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Some specific microRNAs (miRNAs) have been identified to regulate the tumorigenesis of non-small cell lung cancer (NSCLC). MiR-590-5p was found to involve in the carcinogenesis of human cancers. This study aims at exploring the role of miR-590-5p in the pathogenesis of NSCLC.

Patients and methods

The expressions of miR-590-5p and GAB1 were measured by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. The biological functions of miR-590-5p and GAB1 on cell viability and invasion were investigated through MTT and transwell assays. The binding site between miR-590-5p and GAB1 was verified by dual-luciferase reporter gene assay (DLR).

Results

MiR-590-5p expression was downregulated in NSCLC. MiR-590-5p overexpression inhibited the proliferation and invasion of NSCLC cells. Furthermore, miR-590-5p was confirmed to directly target GAB1. GAB1 knockdown had the same effect as overexpression of miR-590-5p in NSCLC. Moreover, overexpression of GAB1 partially reversed the suppressive effect of miR-590-5p on NSCLC.

Conclusions

MiR-590-5p suppressed cell proliferation and invasion of NSCLC by inhibiting GAB1 expression, indicating that miR-590-5p was a suppressive miRNA in NSCLC."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.org/dc/terms/identifier"doi:10.26355/eurrev_201809_15926"xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/author"Ma M."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/author"Wang J.Y."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/author"Wang H.N."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/author"Xu B.B."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/author"Gu Z.F."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/name"Eur Rev Med Pharmacol Sci"xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/pages"5954-5963"xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/title"MicroRNA-590-5p suppresses the proliferation and invasion of non-small cell lung cancer by regulating GAB1."xsd:string
http://purl.uniprot.org/citations/30280777http://purl.uniprot.org/core/volume"22"xsd:string
http://purl.uniprot.org/citations/30280777http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30280777
http://purl.uniprot.org/citations/30280777http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30280777
http://purl.uniprot.org/uniprot/#_B7Z3B9-mappedCitation-30280777http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/#_Q13480-mappedCitation-30280777http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/#_Q8TE87-mappedCitation-30280777http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/#_Q9HA84-mappedCitation-30280777http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/Q9HA84http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/B7Z3B9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/Q8TE87http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30280777
http://purl.uniprot.org/uniprot/Q13480http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30280777