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http://purl.uniprot.org/citations/30280779http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30280779http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To explore the role of microRNA-217 in non-small cell lung cancer (NSCLC) and its underlying mechanism.

Patients and methods

MicroRNA-217 expression in 48 NSCLC tissues and paracancerous tissues were detected by qRT-PCR (quantitative Real-time polymerase chain reaction). The relationship between microRNA-217 expression and prognosis of NSCLC patients was analyzed. Target gene of microRNA-217 was predicted by bioinformatics method and further verified by luciferase reporter gene assay. Cell proliferation, cell cycle and apoptosis were detected after altering microRNA-217 expression in NSCLC cells. The effect of microRNA-217 on regulating PI3K pathway was detected by Western blot.

Results

MicroRNA-217 was downregulated in NSCLC tissues than that of paracancerous tissues. Shorter overall survival (OS) was observed in NSCLC patients with lower expression of microRNA-217 than those with higher expression. Overexpressed microRNA-217 remarkably inhibited proliferation and cell cycle, whereas induced apoptosis of NSCLC cells. AKT3 was screened out to be the target gene of microRNA-217. Western blot results demonstrated that microRNA-217 upregulated AKT3 and PI3K pathway-related genes.

Conclusions

Downregulated microRNA-217 promotes the occurrence and progression of NSCLC through upregulating AKT3 via PI3K pathway."xsd:string
http://purl.uniprot.org/citations/30280779http://purl.org/dc/terms/identifier"doi:10.26355/eurrev_201809_15928"xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/author"Zhang W."xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/author"Qi Y.J."xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/author"Zha W.J."xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/name"Eur Rev Med Pharmacol Sci"xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/pages"5972-5979"xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/title"MicroRNA-217 alleviates development of non-small cell lung cancer by inhibiting AKT3 via PI3K pathway."xsd:string
http://purl.uniprot.org/citations/30280779http://purl.uniprot.org/core/volume"22"xsd:string
http://purl.uniprot.org/citations/30280779http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30280779
http://purl.uniprot.org/citations/30280779http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30280779
http://purl.uniprot.org/uniprot/#_Q56A86-mappedCitation-30280779http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30280779
http://purl.uniprot.org/uniprot/#_Q9Y243-mappedCitation-30280779http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30280779
http://purl.uniprot.org/uniprot/Q9Y243http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30280779
http://purl.uniprot.org/uniprot/Q56A86http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30280779