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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/3031475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3031475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3031475 | http://www.w3.org/2000/01/rdf-schema#comment | "We examined two sets of genes expressed early in the developmental cycle of Dictyostelium discoideum that appear to be regulated by cyclic AMP (cAMP). The transcripts of both sets of genes were not detectable in vegetative cells. During normal development on filter pads, RNA complementary to these genes could be detected at about 2 h, peaked around 6 to 8 h, and decreased gradually thereafter. Expression of these genes upon starvation in shaking culture was stimulated by pulsing the cells with nanomolar levels of cAMP, a condition that mimics the in vivo pulsing during normal aggregation. Expression was inhibited by caffeine or by continuous levels of cAMP, a condition found later in development when in vivo expression of these genes decreased. The inhibition of caffeine could be overcome by pulsing cells with cAMP. These results suggest that the expression is mediated via the cell surface cAMP receptor, but does not require a rise in intracellular cAMP. mRNA from a gene of the second class was induced upon starvation, peaked by 2.5 h of development, and then declined. In contrast to the other genes, its expression was maintained by continuous levels of cAMP and repressed by cAMP pulses. These and other results on a number of classes of developmentally regulated genes indicates that changing levels of cAMP, acting via the cell surface cAMP receptor, are involved in controlling these groups of genes. We also examined the structure and partial sequence of the cAMP pulse-induced genes. The two tandemly duplicated M3 genes were almost continuously homologous over the sequenced portion of the protein-coding region except for a region near the N-terminal end. The two M3 genes had regions of homology in the 5' flanking sequence and showed slight homology to the same regions in gene D2, another cAMP pulse-induced gene. D2 showed extremely significant homology over its entire sequenced length to an acetylcholinesterase. The results presented here and by others suggest that expression of many early genes in D. discoideum is regulated via the cell surface cAMP receptor. We expect that many of these genes may play essential roles in early Dictyostelium development and could code for elements of the cAMP signal transduction pathway involved in aggregation."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.7.1.458-469.1987"xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.7.1.458-469.1987"xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3031475 |
| http://purl.uniprot.org/citations/3031475 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3031475 |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/author | "Firtel R.A."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/author | "Firtel R.A."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/author | "Mann S.K.O."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/author | "Mann S.K.O."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/pages | "458-469"xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/pages | "458-469"xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/title | "Cyclic AMP regulation of early gene expression in Dictyostelium discoideum: mediation via the cell surface cyclic AMP receptor."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/title | "Cyclic AMP regulation of early gene expression in Dictyostelium discoideum: mediation via the cell surface cyclic AMP receptor."xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/3031475 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3031475 |
| http://purl.uniprot.org/citations/3031475 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3031475 |
| http://purl.uniprot.org/uniprot/#_P18142-citation-3031475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/3031475 |
| http://purl.uniprot.org/uniprot/#_P11872-citation-3031475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/3031475 |