| http://purl.uniprot.org/citations/30336314 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30336314 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30336314 | http://www.w3.org/2000/01/rdf-schema#comment | "The present study was performed to explore the underlying molecular mechanism of Cu-induced disorder of lipid metabolism in fish. To this end, adult zebrafish were exposed to three waterborne Cu concentrations (0 (control), 8 and 16 μg Cu/L, respectively) for 60 days. Hepatic Cu content and hepatosomatic index increased after waterborne Cu exposure. H&E and oil red O stainings showed extensive steatosis in the liver of Cu-exposed fish. Cu exposure up-regulated lipogenic enzymes activities of ME, ICDH, 6PGD, G6PD and FAS, but down-regulated CPTI activities. Transcriptomic analysis indicated that lipid metabolism related pathways were significantly enriched in both low-dose and high-dose Cu exposure group. Genes involved in lipogenic process from fatty acid biosynthesis, fatty acid elongation, fatty acid desaturation to glycerolipid biosynthesis were up-regulated by Cu. To elucidate the mechanism, LXRα inhibitor SR9243 and SREBP1 inhibitor fatostatin were used to verify the role of LXRα and SREBP1 in Cu-induced disorder of lipid metabolism. Both SR9243 and fatostatin significantly attenuated the Cu-induced increase of TG accumulation of hepatocytes. Meanwhile, SR9243 significantly attenuated the Cu-induced up-regulation of expression of lipogenic genes (acaca, fas, icdh, dgat1, moat2 and moat3), and fatostatin significantly attenuated the up-regulation of expression of acaca, fas, g6pd, dgat1 and moat2. Enzymes analysis showed both SR9243 and fatostatin blocked the Cu-induced increase of lipogenic enzymes activities. Taken together, our findings highlight the importance of LXRα and SREBP1 in Cu-induced hepatic lipid deposition, which proposed a novel mechanism for elucidating metal element exposure inducing the disorder of lipid metabolism in aquatic vertebrates."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.chemosphere.2018.10.058"xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Luo Z."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Luo Z."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Wu K."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Wu K."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Li D.D."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Li D.D."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Xu Y.H."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Xu Y.H."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Pan Y.X."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Pan Y.X."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Zhuo M.Q."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/author | "Zhuo M.Q."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/name | "Chemosphere"xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/name | "Chemosphere"xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/pages | "370-379"xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/pages | "370-379"xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/title | "SREBP-1 and LXRalpha pathways mediated Cu-induced hepatic lipid metabolism in zebrafish Danio rerio."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/title | "SREBP-1 and LXRalpha pathways mediated Cu-induced hepatic lipid metabolism in zebrafish Danio rerio."xsd:string |
| http://purl.uniprot.org/citations/30336314 | http://purl.uniprot.org/core/volume | "215"xsd:string |