| http://purl.uniprot.org/citations/3033639 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3033639 | http://www.w3.org/2000/01/rdf-schema#comment | "The fraction DE-B obtained by fractionating an extract from rat mammary adenocarcinoma cells on a DEAE-Sephadex column was used for transcribing linear and supercoiled rRNA gene (rDNA). This fraction, which is known to contain RNA polymerase I and essential transcription factors, also contains DNA topoisomerase I activity. Inhibition of this topoisomerase activity by the selective inhibitor camptothecin markedly diminished transcription of supercoiled rDNA, and at a concentration of 150 microM, camptothecin almost completely inhibited DNA topoisomerase I activity and supercoiled rDNA transcription. Addition of exogenous calf thymus DNA topoisomerase I to the sample containing the drug restored the ability of the extract to transcribe supercoiled rDNA. Camptothecin, even at a concentration of 500 microM, had no significant effect on the transcription of linear rDNA. These studies show that relaxation of supercoiled rDNA by DNA topoisomerase I is essential for its transcription. The preferential inhibition of rRNA synthesis in vivo following treatment with camptothecin is probably due to selective camptothecin inhibition of DNA topoisomerase I activity."xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.84.10.3185"xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/author | "Jacob S.T."xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/author | "Garg L.C."xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/author | "DiAngelo S."xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/pages | "3185-3188"xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/title | "Role of DNA topoisomerase I in the transcription of supercoiled rRNA gene."xsd:string |
| http://purl.uniprot.org/citations/3033639 | http://purl.uniprot.org/core/volume | "84"xsd:string |
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