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http://purl.uniprot.org/citations/3041415http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3041415http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3041415http://www.w3.org/2000/01/rdf-schema#comment"Cinnamyl-alcohol dehydrogenase (CAD; EC 1.1.1.195) catalyzes the final step in a branch of phenylpropanoid synthesis specific for production of lignin monomers. We have isolated a full-length cDNA clone encoding CAD, as a molecular marker specific for lignification, by immunoscreening a lambda gt11 library containing cDNAs complementary to mRNA from elicitor-treated cell cultures of bean (Phaseolus vulgaris L.). The clone comprises a single long open reading frame of 1767 base pairs, 31 base pairs of 5' leader, and 152 base pairs of 3' untranslated sequence. The deduced 65-kDa CAD polypeptide has several features that are strongly conserved in alcohol dehydrogenases. Addition of fungal elicitor to cell cultures stimulates CAD transcription, which leads to a remarkably rapid, but transient, accumulation of CAD mRNA, with no detectable lag and maximal levels after 1.5 hr. Southern blot analysis of bean genomic DNA indicates that elicitor-induced CAD is encoded by a single gene. The regulatory significance of the rapid activation of this CAD gene and the possible existence of a second, divergent CAD gene involved in lignification during xylogenesis are discussed."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.org/dc/terms/identifier"doi:10.1073/pnas.85.15.5546"xsd:string
http://purl.uniprot.org/citations/3041415http://purl.org/dc/terms/identifier"doi:10.1073/pnas.85.15.5546"xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Lamb C.J."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Lamb C.J."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Boudet A.M."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Boudet A.M."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Grima-Pettenati J."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Grima-Pettenati J."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Grand C."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Grand C."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Walter M.H."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/author"Walter M.H."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/date"1988"xsd:gYear
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/date"1988"xsd:gYear
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/pages"5546-5550"xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/pages"5546-5550"xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/title"Cinnamyl-alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/title"Cinnamyl-alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor."xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/volume"85"xsd:string
http://purl.uniprot.org/citations/3041415http://purl.uniprot.org/core/volume"85"xsd:string