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http://purl.uniprot.org/citations/30458729http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30458729http://www.w3.org/2000/01/rdf-schema#comment"

Background

Traumatic brain injury (TBI) is a complex condition and remains a prominent public and medical health issue in individuals of all ages. A rapid increase in extracellular glutamate occurs after TBI, leading to glutamate-induced excitotoxicity, which causes neuronal damage and further functional impairments. Although inhibition of glutamate carboxypeptidase II (GCP II) is considered a potential approach for reducing glutamate-induced excitotoxicity after TBI, further detailed evidence regarding its efficacy is required. Therefore, in this study, we examined the differences in the metabolite status between wild-type (WT) and GCP II gene-knockout (KO) mice after TBI using proton magnetic resonance spectroscopy (1H-MRS) and T2-weighted magnetic resonance (MR) imaging with a 7-tesla imaging system, and brain water-content analysis.

Results

Evaluation of glutamate and N-acetylaspartate concentrations revealed a decrease in both levels in the ipsilateral hippocampus at 24 h post-TBI; however, the reduction in glutamate and N-acetylaspartate levels was less marked in GCP II-KO mice than in WT mice (p < 0.05). T2 MR data and brain water-content analysis demonstrated that the extent of cortical edema and brain swelling was less in KO than in WT mice after TBI (p < 0.05).

Conclusion

Using two non-invasive methods, 1H-MRS and T2 MR imaging, as well as in vitro brain-water content measurements, we demonstrated that the mechanism underlying the neuroprotective effects of GCP II-KO against brain swelling in TBI involves changes in glutamate and N-acetylaspartate levels. This knowledge may contribute towards the development of therapeutic strategies for TBI."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.org/dc/terms/identifier"doi:10.1186/s12868-018-0473-5"xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Cao Y."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Ren H."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Zhong C."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Zhang K."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Zhang M."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Wu W."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Zheng D."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/author"Xu S."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/name"BMC Neurosci"xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/pages"75"xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/title"Metabolite differences between glutamate carboxypeptidase II gene knockout mice and their wild-type littermates after traumatic brain injury: a 7-tesla 1H-MRS study."xsd:string
http://purl.uniprot.org/citations/30458729http://purl.uniprot.org/core/volume"19"xsd:string
http://purl.uniprot.org/citations/30458729http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30458729
http://purl.uniprot.org/citations/30458729http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30458729
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http://purl.uniprot.org/uniprot/#_O35409-mappedCitation-30458729http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30458729
http://purl.uniprot.org/uniprot/Q0VDM6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30458729
http://purl.uniprot.org/uniprot/O35409http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/30458729