| http://purl.uniprot.org/citations/30475211 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30475211 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30475211 | http://www.w3.org/2000/01/rdf-schema#comment | "In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNAi in a 'PIN' state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) of eIF2 that additionally promotes stringent AUG selection, but the molecular basis of its dual function was unknown. We present a cryo-electron microscopy (cryo-EM) reconstruction of a yeast 48S pre-initiation complex (PIC), at an overall resolution of 3.0 Å, featuring the N-terminal domain (NTD) of eIF5 bound to the 40S subunit at the location vacated by eIF1. eIF5 interacts with and allows a more accommodated orientation of Met-tRNAi. Substitutions of eIF5 residues involved in the eIF5-NTD/tRNAi interaction influenced initiation at near-cognate UUG codonsin vivo, and the closed/open PIC conformation in vitro, consistent with direct stabilization of the codon:anticodon duplex by the wild-type eIF5-NTD. The present structure reveals the basis for a key role of eIF5 in start-codon selection."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.39273"xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.39273"xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.org/dc/terms/identifier | "doi:10.7554/eLife.39273"xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Kumar R."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Kumar R."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Llacer J.L."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Llacer J.L."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Ramakrishnan V."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Ramakrishnan V."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Kaur S."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Kaur S."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Hussain T."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Hussain T."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Hinnebusch A.G."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Hinnebusch A.G."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Gordiyenko Y."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Gordiyenko Y."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Nanda J.S."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Nanda J.S."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Lorsch J.R."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Lorsch J.R."xsd:string |
| http://purl.uniprot.org/citations/30475211 | http://purl.uniprot.org/core/author | "Saini A.K."xsd:string |