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http://purl.uniprot.org/citations/30502362http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30502362http://www.w3.org/2000/01/rdf-schema#comment"

Heading aims

This topic aims to clarify whether miR-22 directly targets and downregulates the expression of ERα and NK1R-Tr to inhibit the malignant behaviors of breast cancer cells.

Materials and methods

RT-PCR and Western Blotting were used to detect the expression profile of miR-22, NK1R-Tr and ERα. Luciferase reporter assay and CHIP experiment were conducted to investigate the regulation network between miR-22, NK1R-Tr and ERα. MCF-7-ERαI and MDA-MB-231-ERα cell lines were constructed to study the biological behaviors. The SP-NK1R-ERK1/2 signaling pathway was analyzed using Western Blotting. The subcutaneous and metastases tumor models were employed to study the effects of miR-22 on cell proliferation and metastasis of breast cancer cells in vivo.

Key findings

MiR-22 expression level was significantly lower in breast cancerous tissues and cell lines than the adjacent normality, while that of NK1R-Tr increased. The ERα could positively regulate NK1R-Tr expression at DNA level. The descent degree of NK1R-Tr in MCF-7-ERαI cells was far less than that in wild MCF-7 cells, while the findings in MDA-MB-231-ERα cells was more apparent than wild MDA-MB-231 cells. The malignant phenotype was decreased in miR-22 overexpressing cells compared with the wild type. The peak of ERK1/2 phosphorylation was delayed and weakened in miR-22 overexpressing MCF-7 cells, which was agreed with the findings using NK1R-Tr antagonist. The size and number of metastatic tumors declined compared to the controls.

Significance

MiR-22 downregulated the expression of NK1R-Tr and ERα to delay and weaken phosphorylation of ERK1/2 to inhibit proliferation and metastasis of breast cancer cells."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.org/dc/terms/identifier"doi:10.1016/j.lfs.2018.11.057"xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Dong D."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Wang M."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Tong Y."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Zhang F."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Zhou Y."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Yu M."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Shao J."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/author"Niu R."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/name"Life Sci"xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/pages"57-69"xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/title"MicroRNA-22 inhibits proliferation, invasion and metastasis of breast cancer cells through targeting truncated neurokinin-1 receptor and ERalpha."xsd:string
http://purl.uniprot.org/citations/30502362http://purl.uniprot.org/core/volume"217"xsd:string
http://purl.uniprot.org/citations/30502362http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30502362
http://purl.uniprot.org/citations/30502362http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30502362
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http://purl.uniprot.org/uniprot/#_A0A125SXW1-mappedCitation-30502362http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30502362
http://purl.uniprot.org/uniprot/#_Q14268-mappedCitation-30502362http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30502362
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