| http://purl.uniprot.org/citations/30521320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30521320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30521320 | http://www.w3.org/2000/01/rdf-schema#comment | "Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many other Nap proteins. Comparison of CeNap1 with full length and tail-less constructs of Saccharomyces cerevisiae Nap1 uncovers the role of these tails in self-association, histone binding, and Nap competition with DNA for H2A-H2B. We find that the presence of tails influences the stoichiometry of H2A-H2B binding and is required to complete the interactions between H2A-H2B and DNA. The absolute stoichiometry of the Nap protein and H2A-H2B complex is 2:1 or 2:2, with only a very small population of higher-order oligomers occurring at 150 mM NaCl. We also show that H3-H4 binds differently than H2A-H2B and that an (H3-H4)2 tetramer can simultaneously bind two Nap2 protein homodimers."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.8b01033"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.8b01033"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Zhang N."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Zhang N."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Bhattacharyya S."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Bhattacharyya S."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Sarkar P."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Sarkar P."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "D'Arcy S."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "D'Arcy S."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Salvador K."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/author | "Salvador K."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/pages | "108-113"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/pages | "108-113"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/title | "Characterization of Caenorhabditis elegans Nucleosome Assembly Protein 1 Uncovers the Role of Acidic Tails in Histone Binding."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/title | "Characterization of Caenorhabditis elegans Nucleosome Assembly Protein 1 Uncovers the Role of Acidic Tails in Histone Binding."xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/volume | "58"xsd:string |
| http://purl.uniprot.org/citations/30521320 | http://purl.uniprot.org/core/volume | "58"xsd:string |