| http://purl.uniprot.org/citations/30526847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30526847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30526847 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein histidine methylation is a rare post-translational modification of unknown biochemical importance. In vertebrates, only a few methylhistidine-containing proteins have been reported, including β-actin as an essential example. The evolutionary conserved methylation of β-actin H73 is catalyzed by an as yet unknown histidine N-methyltransferase. We report here that the protein SETD3 is the actin-specific histidine N-methyltransferase. In vitro, recombinant rat and human SETD3 methylated β-actin at H73. Knocking-out SETD3 in both human HAP1 cells and in Drosophila melanogaster resulted in the absence of methylation at β-actin H73 in vivo, whereas β-actin from wildtype cells or flies was > 90% methylated. As a consequence, we show that Setd3-deficient HAP1 cells have less cellular F-actin and an increased glycolytic phenotype. In conclusion, by identifying SETD3 as the actin-specific histidine N-methyltransferase, our work pioneers new research into the possible role of this modification in health and disease and questions the substrate specificity of SET-domain-containing enzymes."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.37921"xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.37921"xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Ishikawa T."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Ishikawa T."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Teleman A.A."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Teleman A.A."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Tiebe M."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Tiebe M."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Veiga-da-Cunha M."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Veiga-da-Cunha M."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Vertommen D."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Vertommen D."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Drozak J."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Drozak J."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Grabowska I."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Grabowska I."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Jagielski A.K."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Jagielski A.K."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Kwiatkowski S."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Kwiatkowski S."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Seliga A.K."xsd:string |
| http://purl.uniprot.org/citations/30526847 | http://purl.uniprot.org/core/author | "Seliga A.K."xsd:string |