| http://purl.uniprot.org/citations/30591540 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30591540 | http://www.w3.org/2000/01/rdf-schema#comment | "The 3' ends of metazoan microRNAs (miRNAs) are initially defined by the RNase III enzymes during maturation, but subsequently experience extensive modifications by several enzymatic activities. For example, terminal nucleotidyltransferases (TENTs) elongate miRNAs by adding one or a few nucleotides to their 3' ends, which occasionally leads to differential regulation of miRNA stability or function. However, the catalytic entities that shorten miRNAs and the molecular consequences of such shortening are less well understood, especially in vertebrates. Here, we report that poly(A)-specific ribonuclease (PARN) sculpts the 3' ends of miRNAs in human cells. By generating PARN knockout cells and characterizing their miRNAome, we demonstrate that PARN digests the 3' extensions of miRNAs that are derived from the genome or attached by TENTs, thereby effectively reducing the length of miRNAs. Surprisingly, PARN-mediated shortening has little impact on miRNA stability, suggesting that this process likely operates to finalize miRNA maturation, rather than to initiate miRNA decay. PARN-mediated shortening is pervasive across most miRNAs and appears to be a conserved mechanism contributing to the 3' end formation of vertebrate miRNAs. Our findings add miRNAs to the expanding list of noncoding RNAs whose 3' end formation depends on PARN."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.069633.118"xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/author | "Kim J.H."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/author | "Lee D."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/author | "Park D."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/author | "Park J.H."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/author | "Shin C."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/pages | "388-405"xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/title | "Poly(A)-specific ribonuclease sculpts the 3' ends of microRNAs."xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/30591540 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30591540 |
| http://purl.uniprot.org/citations/30591540 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30591540 |
| http://purl.uniprot.org/uniprot/#_B3KN69-mappedCitation-30591540 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30591540 |
| http://purl.uniprot.org/uniprot/#_B4E0E8-mappedCitation-30591540 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30591540 |
| http://purl.uniprot.org/uniprot/#_O95453-mappedCitation-30591540 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30591540 |
| http://purl.uniprot.org/uniprot/O95453 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30591540 |
| http://purl.uniprot.org/uniprot/B4E0E8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30591540 |
| http://purl.uniprot.org/uniprot/B3KN69 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30591540 |