| http://purl.uniprot.org/citations/30626968 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30626968 | http://www.w3.org/2000/01/rdf-schema#comment | "A key regulated step of transcription is promoter melting by RNA polymerase (RNAP) to form the open promoter complex1-3. To generate the open complex, the conserved catalytic core of the RNAP combines with initiation factors to locate promoter DNA, unwind 12-14 base pairs of the DNA duplex and load the template-strand DNA into the RNAP active site. Formation of the open complex is a multi-step process during which transient intermediates of unknown structure are formed4-6. Here we present cryo-electron microscopy structures of bacterial RNAP-promoter DNA complexes, including structures of partially melted intermediates. The structures show that late steps of promoter melting occur within the RNAP cleft, delineate key roles for fork-loop 2 and switch 2-universal structural features of RNAP-in restricting access of DNA to the RNAP active site, and explain why clamp opening is required to allow entry of single-stranded template DNA into the active site. The key roles of fork-loop 2 and switch 2 suggest a common mechanism for late steps in promoter DNA opening to enable gene expression across all domains of life."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41586-018-0840-5"xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/author | "Campbell E.A."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/author | "Darst S.A."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/author | "Jansen R."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/author | "Boyaci H."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/pages | "382-385"xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/title | "Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding."xsd:string |
| http://purl.uniprot.org/citations/30626968 | http://purl.uniprot.org/core/volume | "565"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P9WGY5-mappedCitation-30626968 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30626968 |
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| http://purl.uniprot.org/uniprot/#_P9WGY9-mappedCitation-30626968 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30626968 |
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| http://purl.uniprot.org/uniprot/#_P9WJG3-mappedCitation-30626968 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30626968 |
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