| http://purl.uniprot.org/citations/30628487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30628487 | http://www.w3.org/2000/01/rdf-schema#comment | "The involvement of several microRNAs (miRs) in the initiation and development of tumors through the suppression of the target gene expression has been highlighted. The aberrant expression of miR-181d-5p and cyclin-dependent kinase inhibitor 3 (CDKN3) in non-small-cell lung cancer (NSCLC) was then screened by microarray analysis. In the present study, we performed a series of in vivo and in vitro experiments for the purpose of investigating their roles in NSCLC and the underlying mechanism. There was a high expression of CDKN3, whereas miR-181d-5p was downregulated in NSCLC. Quantitative RT-PCR, Western blot analysis, and dual-luciferase reporter gene assay further identified that CDKN3 could be negatively regulated by miR-181d-5p. Moreover, the upregulation of miR-181d-5p or silencing of CDKN3 could inactivate the Akt signaling pathway. A549 with the lowest miR-181d-5p and H1975 with the highest CDKN3 among the five NSCLC cell lines (H1299, A549, H1975, NCI-H157, and GLC-82) were adopted for in vitro experiments, in which expression of miR-181d-5p and CDKN3 was altered by transfection of miR-181d-5p mimic/inhibitor or siRNA-targeting CDKN3. Afterwards, cell proliferation, apoptosis, invasion, migration, and angiogenesis, as well as epithelial-mesenchymal transition (EMT), were evaluated, and tumorigenicity was assessed. In addition, an elevation in miR-181d-5p or depletion in CDKN3 led to significant reductions in proliferation, invasion, migration, angiogenesis, EMT, and tumorigenicity of NSCLC cells, coupling with increased cell apoptosis. In conclusion, this study highlights the tumor-suppressive effects of miR-181d-5p on NSCLC via Akt signaling pathway inactivation by suppressing CDKN3, thus providing a promising therapeutic strategy for the treatment of NSCLC."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajplung.00334.2018"xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Wang P."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Zheng L."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Di Y."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Tian Q."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Chen L.L."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Yin X.B."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Xu S.F."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Zhang W.L."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Shi S.S."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/author | "Gao L.M."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/name | "Am J Physiol Lung Cell Mol Physiol"xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/pages | "L918-L933"xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/title | "Tumor-suppressive effects of microRNA-181d-5p on non-small-cell lung cancer through the CDKN3-mediated Akt signaling pathway in vivo and in vitro."xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://purl.uniprot.org/core/volume | "316"xsd:string |
| http://purl.uniprot.org/citations/30628487 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30628487 |
| http://purl.uniprot.org/citations/30628487 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30628487 |
| http://purl.uniprot.org/uniprot/#_A0A0S2KP18-mappedCitation-30628487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30628487 |
| http://purl.uniprot.org/uniprot/#_B7Z1E2-mappedCitation-30628487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30628487 |
| http://purl.uniprot.org/uniprot/#_G3V2J7-mappedCitation-30628487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30628487 |
| http://purl.uniprot.org/uniprot/#_Q86SK4-mappedCitation-30628487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30628487 |