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http://purl.uniprot.org/citations/30656807http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30656807http://www.w3.org/2000/01/rdf-schema#comment"

Background and objective

In obstructive sleep apnoea (OSA), intermittent hypoxia (IH) compromises immune surveillance through the upregulation of the programmed cell death-1 (PD-1) receptor and its ligand (PD-L1). Because the risk of OSA-related cancer depends on age, we assessed PD-L1/PD-1 expression in middle-aged and older patients with OSA as well as in a murine model.

Methods

PD-L1 expression was studied in 41 patients with severe OSA and 40 healthy volunteers (HV), divided into two groups (≤55 and >55 years of age). We used flow cytometry, quantitative PCR (qPCR) and ELISA to determine PD-L1 expression on monocytes and plasma PD-L1 protein levels. Moreover, we analysed PD-L1 expression on an in vivo IH model with old and young mice.

Results

In subjects up to 55 years of age, severe OSA increased PD-L1 surface protein and mRNA level expression on monocytes and soluble-PD-L1 protein concentration in plasma compared to HV. PD-L1 and hypoxia-induced factor (HIF)-1α expression correlated with age in HV, whereas in patients with OSA there was a negative relationship. In the mice exposed to IH, PD-L1 expression on F4/80+ splenocytes was also only increased in young animals. HIF-1α expression was significantly higher in patients with OSA than in HV in subjects up to 55 years of age, while PD-L1 expression in monocytes was related to HIF-1α expression in young patients with OSA.

Conclusion

PD-L1 upregulation in patients with OSA as a consequence of HIF-1α activation occurs mainly in young patients. In older patients with OSA, upregulation was not detected, possibly due to impaired oxygen sensitivity."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.org/dc/terms/identifier"doi:10.1111/resp.13470"xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Feliu J."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Torres M."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Cubillos-Zapata C."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Lopez-Collazo E."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Toledano V."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Aguirre L.A."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Garcia-Sanchez A."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Garcia-Rio F."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Farre R."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Almendros I."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Avendano-Ortiz J."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Casitas R."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Balbas-Garcia C."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/author"Zamarron E."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/name"Respirology"xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/pages"684-692"xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/title"Age-dependent hypoxia-induced PD-L1 upregulation in patients with obstructive sleep apnoea."xsd:string
http://purl.uniprot.org/citations/30656807http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/30656807http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30656807
http://purl.uniprot.org/citations/30656807http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30656807
http://purl.uniprot.org/uniprot/#_Q0GN75-mappedCitation-30656807http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30656807
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