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| http://purl.uniprot.org/citations/30685087 | http://www.w3.org/2000/01/rdf-schema#comment | "53BP1 (TP53-binding protein 1) plays a key role in DNA double-strand break repair by promoting non-homologous end joining (NHEJ) especially during G1 phase of the cell cycle. Nuclear import of 53BP1 is required for proper localization of 53BP1 and maintenance of genome integrity. 53BP1 has a classical bipartite nuclear localization signal (NLS) of sequence 1666-GKRKLITSEEERSPAKRGRKS-1686. Ser1678 within the 53BP1 NLS can be phosphorylated by CDK1/cyclin B, and a phosphomimetic substitution of Ser1678 with aspartate has been shown to negatively regulate nuclear import of 53BP1. Here, the X-ray crystal structures of the nuclear import adaptor importin-α1 bound to the wild-type 53BP1 NLS and the S1678D mutant of 53BP1 NLS are reported at resolutions of 1.9 and 1.7 Å, respectively. In the wild-type structure, not only the two basic clusters of the 53BP1 NLS but also the linker region between the basic clusters made extensive interactions with importin-α1. In the mutant structure, the linker region between the basic clusters in the 53BP1 NLS made fewer interactions with importin-α1 than those observed in the wild-type structure. However, biochemical binding assays using purified proteins showed that the 53BP1 mutation S1678D reduces the binding affinity to importin-α1 only to a modest extent. Implications of these findings for regulatory mechanism of 53BP1 nuclear import are discussed."xsd:string |
| http://purl.uniprot.org/citations/30685087 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2019.01.075"xsd:string |
| http://purl.uniprot.org/citations/30685087 | http://purl.uniprot.org/core/author | "Matsuura Y."xsd:string |
| http://purl.uniprot.org/citations/30685087 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30685087 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/30685087 | http://purl.uniprot.org/core/pages | "236-241"xsd:string |
| http://purl.uniprot.org/citations/30685087 | http://purl.uniprot.org/core/title | "Structural and biochemical characterization of the recognition of the 53BP1 nuclear localization signal by importin-alpha."xsd:string |
| http://purl.uniprot.org/citations/30685087 | http://purl.uniprot.org/core/volume | "510"xsd:string |
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