| http://purl.uniprot.org/citations/30692258 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30692258 | http://www.w3.org/2000/01/rdf-schema#comment | "Arabidopsis seed development involves maternal small interfering RNAs (siRNAs) that induce RNA-directed DNA methylation (RdDM) through the NRPD1-mediated pathway. To investigate their biological functions, we characterized siRNAs in the endosperm and seed coat that were separated by laser-capture microdissection (LCM) in reciprocal genetic crosses with an nrpd1 mutant. We also monitored the spatial-temporal activity of the NRPD1-mediated pathway on seed development using the AGO4:GFP::AGO4 (promoter:GFP::protein) reporter and promoter:GUS sensors of siRNA-mediated silencing. From these approaches, we identified four distinct groups of siRNA loci dependent on or independent of the maternal NRPD1 allele in the endosperm or seed coat. A group of maternally expressed NRPD1-siRNA loci targets endosperm-preferred genes, including those encoding AGAMOUS-LIKE (AGL) transcription factors. Using translational promoter:AGL::GUS constructs as sensors, we demonstrate that spatial and temporal expression patterns of these genes in the endosperm are regulated by the NRPD1-mediated pathway irrespective of complete silencing (AGL91) or incomplete silencing (AGL40) of these target genes. Moreover, altered expression of these siRNA-targeted genes affects seed size. We propose that the corresponding maternal siRNAs could account for parent-of-origin effects on the endosperm in interploidy and hybrid crosses. These analyses reconcile previous studies on siRNAs and imprinted gene expression during seed development."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1807621116"xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/author | "Baulcombe D.C."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/author | "Chen Z.J."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/author | "Zhang C."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/author | "Mosher R.A."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/author | "Kirkbride R.C."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/pages | "2761-2766"xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/title | "Maternal small RNAs mediate spatial-temporal regulation of gene expression, imprinting, and seed development in Arabidopsis."xsd:string |
| http://purl.uniprot.org/citations/30692258 | http://purl.uniprot.org/core/volume | "116"xsd:string |
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