| http://purl.uniprot.org/citations/30692602 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30692602 | http://www.w3.org/2000/01/rdf-schema#comment | "Capillary electrophoresis-mass spectrometry (CE-MS) using a sheathless porous tip interface emerged as an attractive tool in metabolomics thanks to its numerous advantages. One of the main advantages compared to the classical co-axial sheath liquid interface is the increased sensitivity, while maintaining the inherent properties of CE, such as a high separation efficiency and low sample consumption. Specially, the ability to perform nanoliter-based injections from only a few microliters of material in the sample vial makes sheathless CE-MS a well-suited and unique approach for highly sensitive metabolic profiling of limited sample amounts. Therefore, in this work, we demonstrate the utility of sheathless CE-MS for metabolic profiling of biomass-restricted samples, namely for 20 µm-thick tissue sections of kidney from a mouse model of polycystic kidney disease (PKD). The extraction method was designed in such a way to keep a minimum sample-volume in the injection vial, thereby still allowing multiple nanoliter injections for repeatability studies. The developed strategy enabled to differentiate between different stages of PKD and as well changes in a variety of different metabolites could be annotated over experimental groups. These metabolites include carnitine, glutamine, creatine, betaine and creatinine. Overall, this study shows the utility of sheathless CE-MS for biomass-limited metabolomics studies."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-018-37512-8"xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Marina M.L."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Peters D.J.M."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Sanchez-Lopez E."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Mayboroda O.A."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Crego A.L."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Kammeijer G.S.M."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/author | "Ramautar R."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/pages | "806"xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/title | "Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease."xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/30692602 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30692602 |
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