| http://purl.uniprot.org/citations/30705154 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30705154 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30705154 | http://www.w3.org/2000/01/rdf-schema#comment | "During exon ligation, the Saccharomyces cerevisiae spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.aaw5569"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.aaw5569"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Newman A.J."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Newman A.J."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Oubridge C."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Oubridge C."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Fica S.M."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Fica S.M."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Wilkinson M.E."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/author | "Wilkinson M.E."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/pages | "710-714"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/pages | "710-714"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/title | "A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/title | "A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation."xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/volume | "363"xsd:string |
| http://purl.uniprot.org/citations/30705154 | http://purl.uniprot.org/core/volume | "363"xsd:string |