| http://purl.uniprot.org/citations/30716520 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30716520 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30716520 | http://www.w3.org/2000/01/rdf-schema#comment | "Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. To date, no research on immunological functions of fish Pkc-θ has been reported. To address this issue, we cloned the grass carp pkc-θ gene. Phylogenetic and syntenic analysis showed that this gene is the most evolutionarily conserved relative to zebrafish. Real-time quantitative PCR (RT-qPCR) indicated that pkc-θ was expressed at high levels in the gills and spleen of healthy grass carp. Infection with GCRV down regulated pkc-θ expression in the gills and spleen. Gene products that function upstream and downstream of pkc-θ were up regulated in the gill, but were down-regulated in the spleen. These results suggest that direct or indirect targeting of pkc-θ by GCRV may help the virus evade host immune defences in the spleen. Phorbol ester (PMA) treatment of Jurkat T cells induced translocation of grass carp Pkc-θ from the cytoplasm to the plasma membrane. This response to PMA suggests evolutionary conservation of an immune response function in fish Pkc-θ, as well as conservation of its sequence and structural domains. This study expanded our knowledge of the fish PKC gene family, and explored the role of pkc-θ in function of the grass carp immune system, providing new insights which may facilitate further studies of its biological functions."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.fsi.2019.01.057"xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Huang R."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Huang R."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Zhu D."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Zhu D."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Yang C."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Yang C."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Zhu Z."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Zhu Z."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Liao L."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Liao L."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "He L."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "He L."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Mehjabin R."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/author | "Mehjabin R."xsd:string |
| http://purl.uniprot.org/citations/30716520 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |