| http://purl.uniprot.org/citations/30733442 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30733442 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30733442 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/30733442 | http://www.w3.org/2000/01/rdf-schema#comment | "The Elongator complex catalyzes posttranscriptional tRNA modifications by attaching carboxy-methyl (cm5) moieties to uridine bases located in the wobble position. The catalytic subunit Elp3 is highly conserved and harbors two individual subdomains, a radical S-adenosyl methionine (rSAM) and a lysine acetyltransferase (KAT) domain. The details of its modification reaction cycle and particularly the substrate specificity of its KAT domain remain elusive. Here, we present the co-crystal structure of bacterial Elp3 (DmcElp3) bound to an acetyl-CoA analog and compare it to the structure of a monomeric archaeal Elp3 from Methanocaldococcus infernus (MinElp3). Furthermore, we identify crucial active site residues, confirm the importance of the extended N-terminus for substrate recognition and uncover the specific induction of acetyl-CoA hydrolysis by different tRNA species. In summary, our results establish the clinically relevant Elongator subunit as a non-canonical acetyltransferase and genuine tRNA modification enzyme."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-019-08579-2"xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-019-08579-2"xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-019-08579-2"xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Lin T.Y."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Lin T.Y."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Abbassi N.E.H."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Abbassi N.E.H."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Chramiec-Glabik A."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Chramiec-Glabik A."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Glatt S."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Glatt S."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Jemiola-Rzeminska M."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Jemiola-Rzeminska M."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Rozycki J."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Rozycki J."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Zakrzewski K."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/author | "Zakrzewski K."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |
| http://purl.uniprot.org/citations/30733442 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |