| http://purl.uniprot.org/citations/30782842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30782842 | http://www.w3.org/2000/01/rdf-schema#comment | "TFCP2L1 (transcription factor CP2-like 1) is a transcriptional regulator critical for maintaining mouse and human embryonic stem cell (ESC) pluripotency. However, the direct TFCP2L1 target genes are uncharacterized. Here, using gene overexpression, immunoblotting, quantitative real-time PCR, ChIP, and reporter gene assays, we show that TFCP2L1 primarily induces estrogen-related receptor β (Esrrb) expression that supports mouse ESC identity and also selectively enhances Kruppel-like factor 4 (Klf4) expression and thereby promotes human ESC self-renewal. Specifically, we found that in mouse ESCs, TFCP2L1 binds directly to the Esrrb gene promoter and regulates its transcription. Esrrb knockdown impaired Tfcp2l1's ability to induce interleukin 6 family cytokine (leukemia inhibitory factor)-independent ESC self-renewal and to reprogram epiblast stem cells to naïve pluripotency. Conversely, Esrrb overexpression blocked differentiation induced by Tfcp2l1 down-regulation. Moreover, we identified Klf4 as a direct TFCP2L1 target in human ESCs, bypassing the requirement for activin A and basic fibroblast growth factor in short-term human ESC self-renewal. Enforced Klf4 expression recapitulated the self-renewal-promoting effect of Tfcp2l1, whereas Klf4 knockdown eliminated these effects and caused loss of colony-forming capability. These findings indicate that TFCP2L1 functions differently in naïve and primed pluripotency, insights that may help elucidate the different states of pluripotency."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra118.006341"xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Sun H."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Zhang X."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "You Y."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Wang X.'"xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Ding H."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/author | "Ye S.D."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/pages | "6007-6016"xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/title | "The transcription factor TFCP2L1 induces expression of distinct target genes and promotes self-renewal of mouse and human embryonic stem cells."xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://purl.uniprot.org/core/volume | "294"xsd:string |
| http://purl.uniprot.org/citations/30782842 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30782842 |
| http://purl.uniprot.org/citations/30782842 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30782842 |
| http://purl.uniprot.org/uniprot/#_Q53RS7-mappedCitation-30782842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30782842 |
| http://purl.uniprot.org/uniprot/#_Q3UNW5-mappedCitation-30782842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30782842 |
| http://purl.uniprot.org/uniprot/#_Q5JV87-mappedCitation-30782842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30782842 |
| http://purl.uniprot.org/uniprot/#_Q9NZI6-mappedCitation-30782842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30782842 |
| http://purl.uniprot.org/uniprot/Q53RS7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30782842 |
| http://purl.uniprot.org/uniprot/Q3UNW5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30782842 |