| http://purl.uniprot.org/citations/30785395 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30785395 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30785395 | http://www.w3.org/2000/01/rdf-schema#comment | "SETD3 is a member of the SET (Su(var)3-9, Enhancer of zeste, and Trithorax) domain protein superfamily and plays important roles in hypoxic pulmonary hypertension, muscle differentiation, and carcinogenesis. Previously, we identified SETD3 as the actin-specific methyltransferase that methylates the N3 of His73 on β-actin (Kwiatkowski et al., 2018). Here, we present two structures of S-adenosyl-L-homocysteine-bound SETD3 in complex with either an unmodified β-actin peptide or its His-methylated variant. Structural analyses, supported by biochemical experiments and enzyme activity assays, indicate that the recognition and methylation of β-actin by SETD3 are highly sequence specific, and that both SETD3 and β-actin adopt pronounced conformational changes upon binding to each other. In conclusion, this study is the first to show a catalytic mechanism of SETD3-mediated histidine methylation on β-actin, which not only throws light on the protein histidine methylation phenomenon but also facilitates the design of small molecule inhibitors of SETD3."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.43676"xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.43676"xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Wu G."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Wu G."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Xu C."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Xu C."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Yu H."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Yu H."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Tu X."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Tu X."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Min J."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Min J."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Liao S."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Liao S."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Guo Q."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Guo Q."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Drozak J."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Drozak J."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Kwiatkowski S."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Kwiatkowski S."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Tomaka W."xsd:string |
| http://purl.uniprot.org/citations/30785395 | http://purl.uniprot.org/core/author | "Tomaka W."xsd:string |