| http://purl.uniprot.org/citations/30822309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30822309 | http://www.w3.org/2000/01/rdf-schema#comment | "We have used two different live-cell fluorescent protein markers to monitor the formation and localization of double-strand breaks (DSBs) in budding yeast. Using GFP derivatives of the Rad51 recombination protein or the Ddc2 checkpoint protein, we find that cells with three site-specific DSBs, on different chromosomes, usually display 2 or 3 foci that may coalesce and dissociate. This motion is independent of Rad52 and microtubules. Rad51-GFP, by itself, is unable to repair DSBs by homologous recombination in mitotic cells, but is able to form foci and allow repair when heterozygous with a wild type Rad51 protein. The kinetics of formation and disappearance of a Rad51-GFP focus parallels the completion of site-specific DSB repair. However, Rad51-GFP is proficient during meiosis when homozygous, similar to rad51 "site II" mutants that can bind single-stranded DNA but not complete strand exchange. Rad52-RFP and Rad51-GFP co-localize to the same DSB, but a significant minority of foci have Rad51-GFP without visible Rad52-RFP. We conclude that co-localization of foci in cells with 3 DSBs does not represent formation of a homologous recombination "repair center," as the same distribution of Ddc2-GFP foci was found in the absence of the Rad52 protein."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1008001"xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Li K."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Zhou F."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Lee C.S."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Eapen V.V."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Haber J.E."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Tsabar M."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Mazzella A."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/author | "Waterman D.P."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/pages | "e1008001"xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/title | "Live cell monitoring of double strand breaks in S. cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/30822309 | http://purl.uniprot.org/core/volume | "15"xsd:string |
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