| http://purl.uniprot.org/citations/30826286 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30826286 | http://www.w3.org/2000/01/rdf-schema#comment | "Corticotrophin releasing factor (CRF) acts via two family B G-protein-coupled receptors, CRFR1 and CRFR2. Additional subtypes exist due to alternative splicing. CRFR1α is the most widely expressed subtype and lacks a 29-residue insert in the first intracellular loop that is present in CRFR1β. It has been shown previously that co-expression of CRFR1β with receptor activity modifying protein 2 (RAMP2) in HEK 293S cells increased the cell-surface expression of both proteins suggesting a physical interaction as seen with RAMPs and calcitonin receptor-like receptor (CLR). This study investigated the ability of CRFR1α, CRFR1β and CRFR2β to promote cell-surface expression of FLAG-tagged RAMP2. Four different cell-lines were utilised to investigate the effect of varying cellular context; COS-7, HEK 293T, HEK 293S and [ΔCTR]HEK 293 (which lacks endogenous calcitonin receptor). In all cell-lines, CRFR1α and CRFR1β enhanced RAMP2 cell-surface expression. The magnitude of the effect on RAMP2 was dependent on the cell-line ([ΔCTR]HEK 293 > COS-7 > HEK 293T > HEK 293S). RT-PCR indicated this variation may relate to differences in endogenous RAMP expression between cell types. Furthermore, pre-treatment with CRF resulted in a loss of cell-surface FLAG-RAMP2 when it was co-expressed with CRFR1 subtypes. CRFR2β co-expression had no effect on RAMP2 in any cell-line. Molecular modelling suggests that the potential contact interface between the extracellular domains of RAMP2 and CRF receptor subtypes is smaller than that of RAMP2 and CRL, the canonical receptor:RAMP pairing, assuming a physical interaction. Furthermore, a specific residue difference between CRFR1 subtypes (glutamate) and CRFR2β (histidine) in this interface region may impair CRFR2β:RAMP2 interaction by electrostatic repulsion."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamem.2019.02.008"xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Harris M."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Bailey S."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Ladds G."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Simms J."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Harper M.T."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Wheatley M."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Barkan K."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Poyner D."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/author | "Winfield I."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Biomembr"xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/pages | "997-1003"xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/title | "Interactions between RAMP2 and CRF receptors: The effect of receptor subtypes, splice variants and cell context."xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://purl.uniprot.org/core/volume | "1861"xsd:string |
| http://purl.uniprot.org/citations/30826286 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30826286 |
| http://purl.uniprot.org/citations/30826286 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30826286 |
| http://purl.uniprot.org/uniprot/#_O60895-mappedCitation-30826286 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30826286 |
| http://purl.uniprot.org/uniprot/O60895 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30826286 |