| http://purl.uniprot.org/citations/30858373 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30858373 | http://www.w3.org/2000/01/rdf-schema#comment | "Bacterial RNA polymerase employs extra-cytoplasmic function (ECF) σ factors to regulate context-specific gene expression programs. Despite being the most abundant and divergent σ factor class, the structural basis of ECF σ factor-mediated transcription initiation remains unknown. Here, we determine a crystal structure of Mycobacterium tuberculosis (Mtb) RNAP holoenzyme comprising an RNAP core enzyme and the ECF σ factor σH (σH-RNAP) at 2.7 Å, and solve another crystal structure of a transcription initiation complex of Mtb σH-RNAP (σH-RPo) comprising promoter DNA and an RNA primer at 2.8 Å. The two structures together reveal the interactions between σH and RNAP that are essential for σH-RNAP holoenzyme assembly as well as the interactions between σH-RNAP and promoter DNA responsible for stringent promoter recognition and for promoter unwinding. Our study establishes that ECF σ factors and primary σ factors employ distinct mechanisms for promoter recognition and for promoter unwinding."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-019-09096-y"xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/author | "Wang T."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/author | "Zhuang N."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/author | "Fang C."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/pages | "1153"xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/title | "Structural basis for transcription initiation by bacterial ECF sigma factors."xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/30858373 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30858373 |
| http://purl.uniprot.org/citations/30858373 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30858373 |
| http://purl.uniprot.org/uniprot/#_P9WGH9-mappedCitation-30858373 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30858373 |
| http://purl.uniprot.org/uniprot/#_P9WGY5-mappedCitation-30858373 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30858373 |
| http://purl.uniprot.org/uniprot/#_P9WGY7-mappedCitation-30858373 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30858373 |
| http://purl.uniprot.org/uniprot/#_P9WGY9-mappedCitation-30858373 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30858373 |
| http://purl.uniprot.org/uniprot/#_P9WGZ1-mappedCitation-30858373 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30858373 |
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| http://purl.uniprot.org/uniprot/P9WGZ1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30858373 |
| http://purl.uniprot.org/uniprot/P9WGY7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30858373 |
| http://purl.uniprot.org/uniprot/P9WGY5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30858373 |