RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/3086288http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3086288http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/3086288http://www.w3.org/2000/01/rdf-schema#comment"The nucleotide sequence of the bgaB gene, which encodes the thermostable beta-galactosidase I of Bacillus stearothermophilus, and its flanking region was determined. A 2,016-base-pair open reading frame observed was concluded to be for beta-galactosidase I (Mr 78,051) from observations that the amino acid composition of the enzyme and the sequence of 14 amino acids from the amino-terminus of the enzyme coincided with those deduced from this open frame. A 107-base-pair HaeIII-AluI fragment just upstream of the estimated Shine-Dalgarno sequence of the bgaB gene had promoter activity toward cat-86 (chloramphenicol acetyltransferase gene) and produced the enzyme at a level equivalent to 7% of the total cellular protein of B. subtilis. From the base sequence of this DNA region and the transcriptional start site determined by S1 nuclease mapping, the -35 and -10 sequences are estimated to be TTGACA and TAATTT, respectively, which are similar to the consensus sequence of B. subtilis sigma 43 RNA polymerase."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.org/dc/terms/identifier"doi:10.1128/jb.166.3.722-727.1986"xsd:string
http://purl.uniprot.org/citations/3086288http://purl.org/dc/terms/identifier"doi:10.1128/jb.166.3.722-727.1986"xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Hirata H."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Hirata H."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Negoro S."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Negoro S."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Okada H."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Okada H."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Fukazawa T."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/author"Fukazawa T."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/date"1986"xsd:gYear
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/date"1986"xsd:gYear
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/pages"722-727"xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/pages"722-727"xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/title"Structure of a beta-galactosidase gene of Bacillus stearothermophilus."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/title"Structure of a beta-galactosidase gene of Bacillus stearothermophilus."xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/volume"166"xsd:string
http://purl.uniprot.org/citations/3086288http://purl.uniprot.org/core/volume"166"xsd:string
http://purl.uniprot.org/citations/3086288http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/3086288
http://purl.uniprot.org/citations/3086288http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/3086288