| http://purl.uniprot.org/citations/30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30879786 | http://www.w3.org/2000/01/rdf-schema#comment | "Pre-mRNA splicing is executed by the spliceosome. Structural characterization of the catalytically activated complex (B∗) is pivotal for understanding the branching reaction. In this study, we assembled the B∗ complexes on two different pre-mRNAs from Saccharomyces cerevisiae and determined the cryo-EM structures of four distinct B∗ complexes at overall resolutions of 2.9-3.8 Å. The duplex between U2 small nuclear RNA (snRNA) and the branch point sequence (BPS) is discretely away from the 5'-splice site (5'SS) in the three B∗ complexes that are devoid of the step I splicing factors Yju2 and Cwc25. Recruitment of Yju2 into the active site brings the U2/BPS duplex into the vicinity of 5'SS, with the BPS nucleophile positioned 4 Å away from the catalytic metal M2. This analysis reveals the functional mechanism of Yju2 and Cwc25 in branching. These structures on different pre-mRNAs reveal substrate-specific conformations of the spliceosome in a major functional state."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2019.02.006"xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/author | "Shi Y."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/author | "Lei J."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/author | "Yan C."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/author | "Bai R."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/author | "Wan R."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/pages | "339-351.e13"xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/title | "Structures of the Catalytically Activated Yeast Spliceosome Reveal the Mechanism of Branching."xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://purl.uniprot.org/core/volume | "177"xsd:string |
| http://purl.uniprot.org/citations/30879786 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30879786 |
| http://purl.uniprot.org/citations/30879786 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30879786 |
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| http://purl.uniprot.org/uniprot/#_P40204-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |
| http://purl.uniprot.org/uniprot/#_P28004-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |
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| http://purl.uniprot.org/uniprot/#_Q03772-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |
| http://purl.uniprot.org/uniprot/#_Q06217-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |
| http://purl.uniprot.org/uniprot/#_P40968-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |
| http://purl.uniprot.org/uniprot/#_Q12046-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |
| http://purl.uniprot.org/uniprot/#_Q12309-mappedCitation-30879786 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30879786 |