| http://purl.uniprot.org/citations/30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30910922 | http://www.w3.org/2000/01/rdf-schema#comment | "Active transport by renal proximal tubules plays a significant role in drug disposition. During drug development, estimates of renal excretion are essential to dose determination. Kidney bioreactors that reproduce physiologic cues in the kidney, such as flow-induced shear stress, may better predict in vivo drug behavior than do current in vitro models. In this study, we investigated the role of shear stress on active transport of 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP+) by Madin-Darby canine kidney cells exogenously expressing the human organic cation transporters organic cation transporter 2 (OCT2) and multidrug and toxin extrusion protein 1 (MATE1). Cells cultured in a parallel plate under continuous media perfusion formed a tight monolayer with a high barrier to inulin. In response to increasing levels of shear stress (0.2-2 dynes/cm2), cells showed a corresponding increase in transport of ASP+, reaching a maximal 4.2-fold increase at 2 dynes/cm2 compared with cells cultured under static conditions. This transport was inhibited with imipramine, indicating active transport was present under shear stress conditions. Cells exposed to shear stress of 2 dynes/cm2 also showed an increase in RNA expression of both transfected human and endogenous OCT2 (3.7- and 2.0-fold, respectively). Removal of cilia by ammonium sulfate eliminated the effects of shear on ASP+ transport at 0.5 dynes/cm2 with no effect on ASP+ transport under static conditions. These results indicate that shear stress affects active transport of organic cations in renal tubular epithelial cells in a cilia-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.org/dc/terms/identifier | "doi:10.1124/jpet.118.255026"xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Roy S."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Kroetz D.L."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Ferrell N."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Brakeman P.R."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Soler P."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Jayagopal A."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/author | "Fissell W."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/name | "J Pharmacol Exp Ther"xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/pages | "523-530"xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/title | "Apical Shear Stress Enhanced Organic Cation Transport in Human OCT2/MATE1-Transfected Madin-Darby Canine Kidney Cells Involves Ciliary Sensing."xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://purl.uniprot.org/core/volume | "369"xsd:string |
| http://purl.uniprot.org/citations/30910922 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30910922 |
| http://purl.uniprot.org/citations/30910922 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30910922 |
| http://purl.uniprot.org/uniprot/#_B4DDH5-mappedCitation-30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30910922 |
| http://purl.uniprot.org/uniprot/#_B4E3B2-mappedCitation-30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30910922 |
| http://purl.uniprot.org/uniprot/#_B4DX84-mappedCitation-30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30910922 |
| http://purl.uniprot.org/uniprot/#_B4DYV3-mappedCitation-30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30910922 |
| http://purl.uniprot.org/uniprot/#_B4DPA7-mappedCitation-30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30910922 |
| http://purl.uniprot.org/uniprot/#_Q96FL8-mappedCitation-30910922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30910922 |
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| http://purl.uniprot.org/uniprot/B4DDH5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30910922 |