| http://purl.uniprot.org/citations/30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30964885 | http://www.w3.org/2000/01/rdf-schema#comment | "Triple-Negative Breast Cancers (TNBCs) are the most difficult to treat subtype of breast cancer and are often associated with high nuclear expression of Snail and Cathepsin L (Cat L) protease. We have previously shown that Snail can increase Cat L expression/activity in prostate and breast cancer cells. This study investigated the role of CUX1 (a downstream substrate of Cat L) in TNBC. We showed that Cat L and CUX1 were highly expressed in TNBC patient tissue/cell lines, as compared to ER-positive samples, using cBioportal data and western blot/zymography analyses. Additionally, luciferase reporter and chromatin immunoprecipitation assays showed that CUX1 directly bound to estrogen receptor-alpha (ER-α) promoter in MDA-MB-468, a representative TNBC cell line, and that CUX1 siRNA could restore ER-α transcription and protein expression. Furthermore, Snail and CUX1 expression in various TNBC cell lines was inhibited by muscadine grape skin extract (MSKE, a natural grape product rich in anthocyanins) or Cat L inhibitor (Z-FY-CHO) leading to decreased cell invasion and migration. MSKE decreased cell viability and increased expression of apoptotic markers in MDA-MB-468 cells, with no effect on non-tumorigenic MCF10A cells. MSKE also decreased CUX1 binding to ER-α promoter and restored ER-α expression in TNBC cells, while both MSKE and CUX1 siRNA restored sensitivity to estradiol and 4-hydoxytamoxifen as shown by increased cell viability. Therefore, CUX1 activated by Snail-Cat L signaling may contribute to TNBC via ER-α repression, and may be a viable target for TNBC using natural products such as MSKE that targets cancer and not normal cells."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0214844"xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/author | "Hudson T."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/author | "Sweeney J."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/author | "Bowen N."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/author | "Odero-Marah V."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/author | "Burton L.J."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/author | "Hawsawi O."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/pages | "e0214844"xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/title | "CCAAT-displacement protein/cut homeobox transcription factor (CUX1) represses estrogen receptor-alpha (ER-alpha) in triple-negative breast cancer cells and can be antagonized by muscadine grape skin extract (MSKE)."xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/30964885 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30964885 |
| http://purl.uniprot.org/citations/30964885 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW0-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW1-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_Q14268-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXV8-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXV9-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW2-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXW3-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_B6DU68-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |
| http://purl.uniprot.org/uniprot/#_A0A125SXV6-mappedCitation-30964885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30964885 |