| http://purl.uniprot.org/citations/30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30972801 | http://www.w3.org/2000/01/rdf-schema#comment | "The investigation of the interacting proteins with testis-specific calcium-binding protein CBP86-IV (CABYR) was carried out in human spermatozoa. The total RNA from human spermatozoa was extracted, and the ORF sequence of TSCBP86-IV gene was amplified and cloned into expression vector pET-28a. The positive recombinant clones were transformed into Escherichia coli strain BL21 (DE3) to express fusion protein. Then, co-immunoprecipitation (Co-IP) of TSCBP86-IV was performed in BL21 cell lysate expressing CBP86-IV recombinant protein. The immune complex was captured and identified by mass spectrometry. Reverse Co-IP of potential interacting proteins was performed in human sperm cell lysate. The potential protein interactions were confirmed by yeast two-hybrid system. Thirteen proteins were successfully identified in immune complex from E. coli cell lysate. Phosphoglycerate kinase 2 (PGK2) further showed positive results both in reverse Co-IP and yeast two-hybrid experiments and was confirmed to be interacted with TSCBP86-IV in human sperm cells."xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.org/dc/terms/identifier | "doi:10.1111/and.13287"xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/author | "Li D."xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/author | "Shen S."xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/author | "Liang J."xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/name | "Andrologia"xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/pages | "e13287"xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/title | "Testis-specific calcium-binding protein CBP86-IV (CABYR) binds with phosphoglycerate kinase 2 in vitro and in vivo experiment."xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://purl.uniprot.org/core/volume | "51"xsd:string |
| http://purl.uniprot.org/citations/30972801 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30972801 |
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| http://purl.uniprot.org/uniprot/#_E2ITD8-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_E2ITE0-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_E2ITE1-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_E2ITE2-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
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| http://purl.uniprot.org/uniprot/#_A0A024RC21-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_A0A140VJR3-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_B4DXA8-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_O75952-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |
| http://purl.uniprot.org/uniprot/#_G9BQT7-mappedCitation-30972801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30972801 |