| http://purl.uniprot.org/citations/30975767 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30975767 | http://www.w3.org/2000/01/rdf-schema#comment | "The prespliceosome, comprising U1 and U2 small nuclear ribonucleoproteins (snRNPs) bound to the precursor messenger RNA 5' splice site (5'SS) and branch point sequence, associates with the U4/U6.U5 tri-snRNP to form the fully assembled precatalytic pre-B spliceosome. Here, we report cryo-electron microscopy structures of the human pre-B complex captured before U1 snRNP dissociation at 3.3-angstrom core resolution and the human tri-snRNP at 2.9-angstrom resolution. U1 snRNP inserts the 5'SS-U1 snRNA helix between the two RecA domains of the Prp28 DEAD-box helicase. Adenosine 5'-triphosphate-dependent closure of the Prp28 RecA domains releases the 5'SS to pair with the nearby U6 ACAGAGA-box sequence presented as a mobile loop. The structures suggest that formation of the 5'SS-ACAGAGA helix triggers remodeling of an intricate protein-RNA network to induce Brr2 helicase relocation to its loading sequence in U4 snRNA, enabling Brr2 to unwind the U4/U6 snRNA duplex to allow U6 snRNA to form the catalytic center of the spliceosome."xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.aax3289"xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/author | "Charenton C."xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/author | "Wilkinson M.E."xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/pages | "362-367"xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/title | "Mechanism of 5' splice site transfer for human spliceosome activation."xsd:string |
| http://purl.uniprot.org/citations/30975767 | http://purl.uniprot.org/core/volume | "364"xsd:string |
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| http://purl.uniprot.org/uniprot/Q96DI7#attribution-1DA45DE806F2CB1233333C8AD8A24C88 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/30975767 |
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