| http://purl.uniprot.org/citations/30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30978219 | http://www.w3.org/2000/01/rdf-schema#comment | "Primed nephron progenitor cells (NPCs) appear in metanephric mesenchyme by E11.5 and differentiate in response to the inductive WNT9b signal from the ureteric bud. However, the NPC WNT-receptor complex is unknown. We obtained M15 cells from E10.5 mesonephric mesenchyme and systematically analyzed components required for canonical WNT9b-responsiveness. When M15 cells were transfected with a β-catenin luciferase reporter plasmid, exposure to recombinant WNT9b resulted in minimal luciferase activity. We then analyzed mRNA-expression of WNT-pathway components and identified Fzd1-6 and Lrp6 transcripts but not Rspo1. When M15 cells were treated with recombinant RSPO1 the response to transfected WNT9b was augmented 4.8-fold. Co-transfection of M15 cells with Fzd5 (but no other Fzd family member) further increased the WNT9b signal to 16.8-fold and siRNA knockdown of Fzd5 reduced the signal by 52%. Knockdown of Lrp6 resulted in 60% WNT9b signal reduction. We confirmed Fzd5, Lrp6 and Rspo1 mRNA expression in CITED1(+) NPCs from E15.5 embryonic mouse kidney. Thus, while many WNT signaling-pathway components are present by E10.5, optimum responsiveness of E11.5 cap mesenchyme requires that NPCs acquire RSPO1, FZD5 and LRP6."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0215139"xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/author | "Hastie N.D."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/author | "Goodyer P."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/author | "Carroll T.J."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/author | "Karner C.M."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/author | "Dickinson K.K."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/author | "Hammond L.C."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/pages | "e0215139"xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/title | "Molecular determinants of WNT9b responsiveness in nephron progenitor cells."xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/30978219 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30978219 |
| http://purl.uniprot.org/citations/30978219 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30978219 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BQF8-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BR53-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |
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| http://purl.uniprot.org/uniprot/#_Q149J3-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |
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| http://purl.uniprot.org/uniprot/#_Q2TBA6-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |
| http://purl.uniprot.org/uniprot/#_Q8BKS3-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |
| http://purl.uniprot.org/uniprot/#_Q8BKU9-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |
| http://purl.uniprot.org/uniprot/#_Q8BLL2-mappedCitation-30978219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30978219 |