| http://purl.uniprot.org/citations/30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30984535 | http://www.w3.org/2000/01/rdf-schema#comment | "Transthyretin (TTR) is a transporter for thyroid hormone and retinol binding protein that has recently been reported to have proteolytic activity against certain substrates, including amidated neuropeptide Y (NPY). However, the proteolytic activity of TTR towards NPY is not fully understood. Here, we used fluorescence-based assays to determine the catalytic kinetics of human TTR towards human amidated NPY. The Michaelis constant (KM) and catalytic efficiency (kcat/KM) of TTR proteolysis were 15.88 ± 0.44 μm and 687 081 ± 35 692 m -1·s-1, respectively. In addition, we demonstrated an effect of the C-terminal sequence of TTR. When the C-terminal sequence of TTR was made more hydrophobic, the KM and kcat/KM changed to 12.87 ± 0.22 μm and 983 755 ± 18 704 m -1·s-1, respectively. Our results may be useful for the development of TTR as a therapeutic agent with low risk of the undesirable symptoms that develop from amidated NPY, and for further improvement of the kcat/KM of TTR."xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.org/dc/terms/identifier | "doi:10.1002/2211-5463.12604"xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/author | "Prapunpoj P."xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/author | "Leelawatwattana L."xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/author | "Tangthavewattana S."xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/name | "FEBS Open Bio"xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/pages | "594-604"xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/title | "The hydrophobic C-terminal sequence of transthyretin affects its catalytic kinetics towards amidated neuropeptide Y."xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/30984535 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/30984535 |
| http://purl.uniprot.org/citations/30984535 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/30984535 |
| http://purl.uniprot.org/uniprot/#_A4D158-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_A0A7R6RQ48-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_A6XMH1-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_E9KL36-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_Q53WY6-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_P02766-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
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| http://purl.uniprot.org/uniprot/#_Q8TDB1-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_Q8TDB2-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/#_Q8TDB3-mappedCitation-30984535 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/A4D158 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30984535 |
| http://purl.uniprot.org/uniprot/P01303 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/30984535 |