| http://purl.uniprot.org/citations/31019301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31019301 | http://www.w3.org/2000/01/rdf-schema#comment | "The turnover of the intestinal epithelium is driven by multipotent LGR5+ crypt-base columnar cells (CBCs) located at the bottom of crypt zones1. However, CBCs are lost following injury, such as irradiation2, but the intestinal epithelium is nevertheless able to recover3. Thus, a second population of quiescent '+4' cells, or reserve stem cells (RSCs), has previously been proposed to regenerate the damaged intestine4-7. Although CBCs and RSCs were thought to be mutually exclusive4,8, subsequent studies have found that LGR5+ CBCs express RSC markers9 and that RSCs were dispensable-whereas LGR5+ cells were essential-for repair of the damaged intestine3. In addition, progenitors of absorptive enterocytes10, secretory cells11-15 and slow cycling LGR5+ cells16 have been shown to contribute to regeneration whereas the transcriptional regulator YAP1, which is important for intestinal regeneration, was suggested to induce a pro-survival phenotype in LGR5+ cells17. Thus, whether cellular plasticity or distinct cell populations are critical for intestinal regeneration remains unknown. Here we applied single-cell RNA sequencing to profile the regenerating mouse intestine and identified a distinct, damage-induced quiescent cell type that we term the revival stem cell (revSC). revSCs are marked by high clusterin expression and are extremely rare under homoeostatic conditions, yet give rise-in a temporal hierarchy-to all the major cell types of the intestine, including LGR5+ CBCs. After intestinal damage by irradiation, targeted ablation of LGR5+ CBCs, or treatment with dextran sodium sulfate, revSCs undergo a YAP1-dependent transient expansion, reconstitute the LGR5+ CBC compartment and are required to regenerate a functional intestine. These studies thus define a unique stem cell that is mobilized by damage to revive the homoeostatic stem cell compartment and regenerate the intestinal epithelium."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41586-019-1154-y"xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Kumar S."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Shen J."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Chan K."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Wrana J.L."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Fink M."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Trcka D."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Gregorieff A."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Ghoshal B."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Ayyaz A."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Barutcu S."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Gosio J."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Ouladan S."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/author | "Sangiorgi B."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/pages | "121-125"xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/title | "Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell."xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://purl.uniprot.org/core/volume | "569"xsd:string |
| http://purl.uniprot.org/citations/31019301 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31019301 |
| http://purl.uniprot.org/citations/31019301 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31019301 |
| http://purl.uniprot.org/uniprot/#_E9Q8Y5-mappedCitation-31019301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31019301 |
| http://purl.uniprot.org/uniprot/#_E9Q9B8-mappedCitation-31019301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31019301 |