| http://purl.uniprot.org/citations/31037730 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31037730 | http://www.w3.org/2000/01/rdf-schema#comment | "The CRISPR-Cas9 technology has been a powerful means to manipulate the genome in a wide range of organisms. A series of GFP knocked-in (GFPKI ) Drosophila strains have been generated through CRISPR-Cas9-induced double strand breaks coupled with homology-directed repairs in the presence of donor plasmids. They visualized specific cell types or intracellular structures in both fixed and live specimen. We provide a rapid and efficient strategy to identify KI lines. This method requires neither co-integration of a selection marker nor prior establishment of sgRNA-expressing transgenic lines. The injection of the mixture of a sgRNA/Cas9 expression plasmid and a donor plasmid into cleavage stage embryos efficiently generated multiple independent KI lines. A PCR-based selection allows to identify KI fly lines at the F1 generation (approximately 4 weeks after injection). These GFPKI strains have been deposited in the Kyoto Drosophila stock center, and made freely available to researchers at non-profit organizations. Thus, they will be useful resources for Drosophila research."xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.org/dc/terms/identifier | "doi:10.1111/dgd.12607"xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/author | "Nakamura A."xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/author | "Hanyu-Nakamura K."xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/author | "Yoshitani T."xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/author | "Kina H."xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/name | "Dev Growth Differ"xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/pages | "265-275"xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/title | "Rapid and efficient generation of GFP-knocked-in Drosophila by the CRISPR-Cas9-mediated genome editing."xsd:string |
| http://purl.uniprot.org/citations/31037730 | http://purl.uniprot.org/core/volume | "61"xsd:string |
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