| http://purl.uniprot.org/citations/31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31050353 | http://www.w3.org/2000/01/rdf-schema#comment | "Nitrogen deficiency (-N) in plants triggers leaf senescence which is regulated by the transcription factor ORE1. Little is known about post-translational regulation of ORE1 in this process. Here, we show that UBP12/UBP13 (ubiquitin-specific protease 12/13) antagonize the action of NLA (nitrogen limitation adaptation) E3 ligase to maintain ORE1 homeostasis. In vitro pull-down and in vivo co-immunoprecipitation assays demonstrated specific binding between UBP12/UBP13 and ORE1. We further analyzed in various genotypes total Chl content and expression levels of senescence-related genes under -N conditions. We found that UBP12/UBP13 can deubiquitinate polyubiquitinated ORE1 in vitro and increase the stability of ORE1 in vivo in MG132/cycloheximide-chase experiments. Plants overexpressing UBP12/UBP13 display accelerated leaf senescence which is reversed by the ore1 mutation. By contrast, the senescence phenotype of plants overexpressing ORE1 is exacerbated by UBP12/UBP13 overexpression. The expression of senescence-related genes tracks the senescence phenotype. ORE1 protein levels can be elevated by UBP12/UBP13 overexpression but decreased in ubp12-2w/13-3. In conclusion, UBP12/UBP13 deubiquitinate ORE1 to stabilize this transcription factor and promote its activity as a positive regulator for leaf senescence under -N conditions. Our study shows that UBP12/UBP13 counteracts the effect of NLA E3 ligase to accelerate leaf senescence under nitrogen starvation."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.org/dc/terms/identifier | "doi:10.1111/nph.15879"xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/author | "Chua N.H."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/author | "Park S.H."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/author | "Jeong J.S."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/author | "Park B.S."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/author | "Seo J.S."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/name | "New Phytol"xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/pages | "1447-1460"xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/title | "Arabidopsis ubiquitin-specific proteases UBP12 and UBP13 shape ORE1 levels during leaf senescence induced by nitrogen deficiency."xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://purl.uniprot.org/core/volume | "223"xsd:string |
| http://purl.uniprot.org/citations/31050353 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31050353 |
| http://purl.uniprot.org/citations/31050353 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31050353 |
| http://purl.uniprot.org/uniprot/#_A0A178VEE0-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_F4K3X1-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_A0A1P8AR01-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_A0A384L9E9-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_A0A654G6C9-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_F4J7I2-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_Q84WU2-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_Q9SRX9-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_Q9FPT1-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |
| http://purl.uniprot.org/uniprot/#_Q9FKA0-mappedCitation-31050353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31050353 |