| http://purl.uniprot.org/citations/31058311 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31058311 | http://www.w3.org/2000/01/rdf-schema#comment | "In Azospirillum brasilense, a gram-negative nitrogen-fixing bacterium, l-arabinose is converted to α-ketoglutarate through a nonphosphorylative metabolic pathway. In the first step in the pathway, l-arabinose is oxidized to l-arabino-γ-lactone by NAD(P)-dependent l-arabinose 1-dehydrogenase (AraDH) belonging to the glucose-fructose oxidoreductase/inositol dehydrogenase/rhizopine catabolism protein (Gfo/Idh/MocA) family. Here, we determined the crystal structures of apo- and NADP-bound AraDH at 1.5 and 2.2 Å resolutions, respectively. A docking model of l-arabinose and NADP-bound AraDH and structure-based mutational analyses suggest that Lys91 or Asp169 serves as a catalytic base and that Glu147, His153, and Asn173 are responsible for substrate recognition. In particular, Asn173 may play a role in the discrimination between l-arabinose and d-xylose, the C4 epimer of l-arabinose."xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.org/dc/terms/identifier | "doi:10.1002/1873-3468.13424"xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/author | "Watanabe Y."xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/author | "Watanabe S."xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/author | "Watanabe Y.'"xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/author | "Iga C."xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/name | "FEBS Lett"xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/pages | "1257-1266"xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/title | "Structural insights into the catalytic and substrate recognition mechanisms of bacterial l-arabinose 1-dehydrogenase."xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://purl.uniprot.org/core/volume | "593"xsd:string |
| http://purl.uniprot.org/citations/31058311 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31058311 |
| http://purl.uniprot.org/citations/31058311 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31058311 |
| http://purl.uniprot.org/uniprot/#_Q53TZ2-mappedCitation-31058311 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31058311 |
| http://purl.uniprot.org/uniprot/Q53TZ2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31058311 |