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http://purl.uniprot.org/citations/31081094http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31081094http://www.w3.org/2000/01/rdf-schema#comment"

Objective

MicroRNAs (miRNAs) are endogenous, non-coding small RNAs, which play an important part in regulating organismal and pathological processes. Previous studies have shown that miRNA-199 acts as a tumor suppressor gene. However, we aimed to explore the characteristics and function of miRNA-199 in lung cancer (LCa), so as to further study its relationship with clinicopathological parameters and prognosis.

Patients and methods

Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect miRNA-199 expression in 75 pairs of LCa tissues and normal adjacent tissues. In addition, the relationship between miRNA-199 expression and pathological features along with the prognosis of LCa patients were investigated. Besides, the expression level of miRNA-199 in LCa cells was further validated by qRT-PCR. In addition, miRNA-199 overexpression expression model was constructed in LCa cell lines H1299 and SPCA1. Cell counting kit-8 (CCK-8), cell cloning experiments, transwell invasion and migration assays were performed to analyze the effect of miRNA-199 on the biological function of LCa cells. Finally, the potential mechanism was explored using Western blot.

Results

qRT-PCR results displayed that the expression level of miRNA-199 in LCa tissues was significantly lower than that of the normal tissues. Compared with patients with high miRNA-199 expression, patients with lowly-expressed miRNA-199 had higher rates of lymph node metastases and distant metastases, and their overall survival rates were lower. In addition, the proliferation, invasion and metastasis of the miRNA-199 overexpression group were significantly increased than that in the negative control group. Western Blot results showed that the expression of key proteins in the EMT pathway, such as N-cadherin, Vimentin, β-catenin and MMP9 significantly increased in miRNA-199 overexpression group. Moreover, we also found that miRNA-199 and RGS17 have mutual regulation, which inhibited the malignant progression of LCa.

Conclusions

miRNA-199 expression was down-regulated in LCa and was significantly associated with LCa stage, distant metastasis, and poor prognosis. Besides, miRNA-199 may inhibit the malignant progression of LCa by interacting with RGS17."xsd:string
http://purl.uniprot.org/citations/31081094http://purl.org/dc/terms/identifier"doi:10.26355/eurrev_201904_17703"xsd:string
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/author"Su W.Z."xsd:string
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/author"Ren L.F."xsd:string
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/name"Eur Rev Med Pharmacol Sci"xsd:string
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/pages"3390-3400"xsd:string
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/title"MiRNA-199 inhibits malignant progression of lung cancer through mediating RGS17."xsd:string
http://purl.uniprot.org/citations/31081094http://purl.uniprot.org/core/volume"23"xsd:string
http://purl.uniprot.org/citations/31081094http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31081094
http://purl.uniprot.org/citations/31081094http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31081094
http://purl.uniprot.org/uniprot/#_Q9UGC6-mappedCitation-31081094http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31081094
http://purl.uniprot.org/uniprot/Q9UGC6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31081094